Detailed information of TA system TA227123

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 109877..110395
• Replicon: plasmid pLP2
• Accession: NZ_CP088910
• Organism: Rhodococcus qingshengii strain VT6

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: Q6XN60
• Locus tag: LRL17_RS34125
• Protein ID: WP_011133429.1
• Coordinates: 109877..110179 (-)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A8E3P5W3
• Locus tag: LRL17_RS34130
• Protein ID: WP_064444958.1
• Coordinates: 110180..110395 (-)
• Length: 72 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LRL17_RS34110 (LRL17_34110)	106801..106908	-	108	Protein_101	ArsR family transcriptional regulator	-
LRL17_RS34115 (LRL17_34115)	107081..107878	-	798	WP_231783884.1	hypothetical protein	-
LRL17_RS34120 (LRL17_34120)	107878..109848	-	1971	WP_231783885.1	hypothetical protein	-
LRL17_RS34125 (LRL17_34125)	109877..110179	-	303	WP_011133429.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
LRL17_RS34130 (LRL17_34130)	110180..110395	-	216	WP_064444958.1	hypothetical protein	Antitoxin
LRL17_RS34135 (LRL17_34135)	110535..110723	-	189	WP_082920751.1	hypothetical protein	-
LRL17_RS34140 (LRL17_34140)	110922..112217	-	1296	WP_149357432.1	AAA family ATPase	-
LRL17_RS34145 (LRL17_34145)	112288..113475	-	1188	WP_149357433.1	hypothetical protein	-
LRL17_RS34150 (LRL17_34150)	113472..113666	-	195	WP_231783855.1	hypothetical protein	-
LRL17_RS34155 (LRL17_34155)	113663..114901	-	1239	WP_231783886.1	VWA-like domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..188969	188969

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (8-83)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 10470.01 Da
• Isoelectric Point: 4.7317

>T227123 WP_011133429.1 NZ_CP088910:c110179-109877 [Rhodococcus qingshengii]
VRPIHDAQLGGLCPVLVLTRELVRPHLGTVTVAPIRSEGRGLSTELSLGEGNGVEDGSVVDCDGVTTIPVSALGVNRGFL
LPAQEVLLSRVLRSAFDLES

Nucleotide

• Length: 303 bp

>T227123 NZ_CP122722:1165918-1166229 [Escherichia coli]
ATGCACATCATTTCTAAAGCGCCATTTGAAGAGTCTGCGCGTAAATATCCTAACGACGCTCTGGCTCTTCAGGCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACACCAGAAGAAATGCGAACGGCGTTTCCCAACCTCGACAATTTTAGATATC
GCAACAAATGGTACGTTCTGGATGTCGGCGGGAACAATCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACGAATCACGCAGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 72 a.a.
• Molecular weight: 7670.69 Da
• Isoelectric Point: 8.9167

>AT227123 WP_064444958.1 NZ_CP088910:c110395-110180 [Rhodococcus qingshengii]
MSTQIAVRLPEDIVAFLDSEVSGGRAKSRAQVVLRALEREQRRQLAVRDVELLSDGAGSDVNIAAAHGKKN

Nucleotide

• Length: 216 bp

>AT227123 NZ_CP122722:1166226-1166645 [Escherichia coli]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAGTATGAAAACAACAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCGGCAATGCCGGTT
GGCGTGGCGTTACTTCGCACGTTGATTGATCAACATAATCTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGTCAGATTTTGTCTGGACAACGATCCCTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Toxin

• AlphaFold DB: Q6XN60