Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 267678..268243 Replicon plasmid pLP1
Accession NZ_CP088909
Organism Rhodococcus qingshengii strain VT6

Toxin (Protein)


Gene name mazF Uniprot ID C3JNM9
Locus tag LRL17_RS32340 Protein ID WP_003943396.1
Coordinates 267887..268243 (+) Length 119 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID C3JNM8
Locus tag LRL17_RS32335 Protein ID WP_003943374.1
Coordinates 267678..267890 (+) Length 71 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LRL17_RS32315 (LRL17_32315) 262755..263846 - 1092 WP_003941566.1 hypothetical protein -
LRL17_RS32320 (LRL17_32320) 264375..265274 - 900 WP_231783826.1 ISL3 family transposase -
LRL17_RS32325 (LRL17_32325) 265285..266350 - 1066 Protein_254 IS630 family transposase -
LRL17_RS32330 (LRL17_32330) 266363..266779 - 417 WP_073513843.1 transposase family protein -
LRL17_RS32335 (LRL17_32335) 267678..267890 + 213 WP_003943374.1 ribbon-helix-helix domain-containing protein Antitoxin
LRL17_RS32340 (LRL17_32340) 267887..268243 + 357 WP_003943396.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
LRL17_RS32345 (LRL17_32345) 268441..270117 + 1677 WP_231783827.1 IS21 family transposase -
LRL17_RS32350 (LRL17_32350) 270132..270803 + 672 Protein_259 IS21-like element helper ATPase IstB -
LRL17_RS32355 (LRL17_32355) 270850..272250 - 1401 WP_231783867.1 integrase -
LRL17_RS32360 (LRL17_32360) 272140..272484 + 345 WP_073513720.1 hypothetical protein -
LRL17_RS32365 (LRL17_32365) 272501..272818 - 318 WP_003943376.1 helix-turn-helix transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..501672 501672
- inside IScluster/Tn - - 265285..270836 5551


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-117)

Antitoxin

Ribbon-helix-helix protein, CopG

(5-39)


Sequences

Toxin        


Download         Length: 119 a.a.        Molecular weight: 12844.58 Da        Isoelectric Point: 6.8817

>T227121 WP_003943396.1 NZ_CP088909:267887-268243 [Rhodococcus qingshengii]
MIRRAHIHWVDLDPAQGSEAAKRRPAVVVSNDRANATAENLGRGVITVVPLTTNTAHVFPFQVIVESDESGLARASKAQA
EQVRSVSVRRLDPEPVGKVGMETFAALEDALRLHLDLQ

Download         Length: 357 bp

>T227121 NZ_CP122722:933774-934181 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 71 a.a.        Molecular weight: 7682.43 Da        Isoelectric Point: 4.1824

>AT227121 WP_003943374.1 NZ_CP088909:267678-267890 [Rhodococcus qingshengii]
MTTKFTISLPDEDAHLLDEYVRDHGLRGRSAGIRAALRLLSTSGLERDYAAAFAEGDVDEVWDAVTADTE

Download         Length: 213 bp

>AT227121 NZ_CP122722:933527-933793 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1F2PUJ5

Antitoxin

Source ID Structure
AlphaFold DB A0A1F2PWJ9

References