Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 159830..160365 Replicon plasmid pLP1
Accession NZ_CP088909
Organism Rhodococcus qingshengii strain VT6

Toxin (Protein)


Gene name mazF Uniprot ID A0A1Q4JD08
Locus tag LRL17_RS31800 Protein ID WP_024488082.1
Coordinates 160066..160365 (+) Length 100 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A7Z6WKL8
Locus tag LRL17_RS31795 Protein ID WP_024488083.1
Coordinates 159830..160066 (+) Length 79 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LRL17_RS31775 (LRL17_31775) 155626..155778 + 153 WP_007733689.1 hypothetical protein -
LRL17_RS31780 (LRL17_31780) 156237..157382 + 1146 Protein_144 IS3 family transposase -
LRL17_RS31785 (LRL17_31785) 157668..158642 + 975 WP_024488085.1 DUF2637 domain-containing protein -
LRL17_RS31790 (LRL17_31790) 158752..159378 + 627 WP_231783801.1 TetR/AcrR family transcriptional regulator -
LRL17_RS31795 (LRL17_31795) 159830..160066 + 237 WP_024488083.1 YlcI/YnfO family protein Antitoxin
LRL17_RS31800 (LRL17_31800) 160066..160365 + 300 WP_024488082.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
LRL17_RS31805 (LRL17_31805) 160394..162244 + 1851 WP_231783802.1 hypothetical protein -
LRL17_RS31810 (LRL17_31810) 162244..163041 + 798 WP_021334701.1 hypothetical protein -
LRL17_RS31815 (LRL17_31815) 163160..164230 - 1071 WP_073513760.1 PDDEXK nuclease domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..501672 501672


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-92)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 100 a.a.        Molecular weight: 10401.98 Da        Isoelectric Point: 4.6942

>T227120 WP_024488082.1 NZ_CP088909:160066-160365 [Rhodococcus qingshengii]
MRPIHEASLDTVRPVLVLTREMVRPHLGTVTVAPITGEIRGLSTEVSVSAANGLSADAVVSCDHITTIPVSALGNIIGYL
LPAQEESLSIAVRAAFDLE

Download         Length: 300 bp

>T227120 NZ_CP122722:c829607-829230 [Escherichia coli]
ATGAACACATTACCCGACACACACGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCATTACGGCCTCACGCTAAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGCCGGGATTC
AGCAGCTGTACCCACTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCCCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATGACATTACCCAGGGGAAGCATCCGGAGGCGAAACAATGA

Antitoxin


Download         Length: 79 a.a.        Molecular weight: 8782.86 Da        Isoelectric Point: 6.8809

>AT227120 WP_024488083.1 NZ_CP088909:159830-160066 [Rhodococcus qingshengii]
MSTQIAVRLPDEIVEFLDDEVREHRAASRASLVLRALERERRRQIAARDAEILSAATSEHDLSPLAHHTVKRQPTGST

Download         Length: 237 bp

>AT227120 NZ_CP122722:c830065-829697 [Escherichia coli]
GTGTCAGACACACTCTCCGGGACAACGCATCCCGATGACAATCACGACCGTCCCTGGTGGGGGCTACCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAAGGGAACCGGTTGCATTACCTTGCAGACCGTGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCGTACCATCTGGACCAGGCCTTTCCGCTACTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGTTG
TGGCTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Q4JD08

Antitoxin

Source ID Structure
AlphaFold DB A0A7Z6WKL8

References