Detailed information of TA system TA227095

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 2377..2965
• Replicon: plasmid pDETABR21-1
• Accession: NZ_CP088896
• Organism: Acinetobacter baumannii strain DETAB-R21

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: -
• Locus tag: LRX86_RS18455
• Protein ID: WP_096877510.1
• Coordinates: 2377..2664 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: N9M5I3
• Locus tag: LRX86_RS18460
• Protein ID: WP_001983304.1
• Coordinates: 2651..2965 (+)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LRX86_RS18440 (LRX86_18440)	204..446	+	243	WP_032011146.1	hypothetical protein	-
LRX86_RS18445 (LRX86_18445)	570..716	-	147	WP_139841515.1	PTD012 family protein	-
LRX86_RS18450 (LRX86_18450)	1177..2061	-	885	WP_032011147.1	nucleotide-binding protein	-
LRX86_RS18455 (LRX86_18455)	2377..2664	+	288	WP_096877510.1	BrnT family toxin	Toxin
LRX86_RS18460 (LRX86_18460)	2651..2965	+	315	WP_001983304.1	BrnA antitoxin family protein	Antitoxin
LRX86_RS18465 (LRX86_18465)	3093..5504	+	2412	WP_096877509.1	TonB-dependent receptor ZnuD2	-
LRX86_RS18470 (LRX86_18470)	5809..6270	+	462	WP_000761346.1	DIP1984 family protein	-
LRX86_RS18475 (LRX86_18475)	6616..6921	-	306	WP_062911967.1	hypothetical protein	-
LRX86_RS18480 (LRX86_18480)	6984..7391	-	408	WP_063099733.1	type II toxin-antitoxin system VapC family toxin	-
LRX86_RS18485 (LRX86_18485)	7391..7630	-	240	WP_063099732.1	type II toxin-antitoxin system VapB family antitoxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..12709	12709

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (41-96)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11329.75 Da
• Isoelectric Point: 5.6762

>T227095 WP_096877510.1 NZ_CP088896:2377-2664 [Acinetobacter baumannii]
MEQYFEWDEAKNRKNQKKHDVSFETASLIFEDPLRISIQDRYTNGEERWQTIGRVKGVLMLLVAHTIFDEDDCEIIRIIS
ARQVTKAERNKYEHG

Nucleotide

• Length: 288 bp

>T227095 NZ_CP122719:c827344-826967 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCACGCCCGTCTCCGGTTGAAATCTGGCAGACACTGCTCAC
CCGACTGCTGGATCAGCACTACGGCCTTACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGCGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTACGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGTTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCACGCCGGGCAACCGGCCTGATGACACGCGA
CAACTACAGAACGGTAAATAACATTACCCTGGGTAAACATCCGGGGGCGAAACAATGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 12208.84 Da
• Isoelectric Point: 7.2026

>AT227095 WP_001983304.1 NZ_CP088896:2651-2965 [Acinetobacter baumannii]
MSMVRYSHKELNEKFGEKQDAEIQRLLAKGTVPDDQLDLSDIPEITDWSNAVRQNQFYRPVKQQTSIRLDADVLAWFKAQ
GKGYQTRMNEILRDAMLKELKNHQ

Nucleotide

• Length: 315 bp

>AT227095 NZ_CP122719:c827802-827434 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACGCTTCCCGACGACAATAACGACCGCACCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTTGGTGCCCGTCTGGTACAGGAAGGCAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGGCGGT
TCAGCGACGCGGATTCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGTTG
TGGCTACGTTTATATGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Antitoxin

• AlphaFold DB: N9M5I3