Detailed information of TA system TA022709

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 27168..27803
• Replicon: plasmid pAQ6
• Accession: NC_010480
• Organism: Synechococcus sp. PCC 7002

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: B1XR29
• Locus tag: SYNPCC7002_RS14815
• Protein ID: WP_012308571.1
• Coordinates: 27168..27563 (-)
• Length: 132 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: B1XR30
• Locus tag: SYNPCC7002_RS14820
• Protein ID: WP_012308572.1
• Coordinates: 27564..27803 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SYNPCC7002_RS14785	24955..25269	+	315	WP_012308565.1	type II toxin-antitoxin system HigB family toxin	-
SYNPCC7002_RS14790	25282..25668	+	387	WP_012308566.1	helix-turn-helix domain-containing protein	-
SYNPCC7002_RS14795	25703..26128	+	426	WP_012308567.1	XisH family protein	-
SYNPCC7002_RS14800	26116..26451	+	336	WP_012308568.1	XisI protein	-
SYNPCC7002_RS14805	26507..26758	+	252	WP_012308569.1	hypothetical protein	-
SYNPCC7002_RS14810	26745..27167	+	423	WP_012308570.1	PIN domain-containing protein	-
SYNPCC7002_RS14815	27168..27563	-	396	WP_012308571.1	type II toxin-antitoxin system VapC family toxin	Toxin
SYNPCC7002_RS14820	27564..27803	-	240	WP_012308572.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
SYNPCC7002_RS14825	27841..31176	+	3336	WP_012308573.1	DEAD/DEAH box helicase family protein	-
SYNPCC7002_RS14830	31342..31797	+	456	WP_012308574.1	hypothetical protein	-
SYNPCC7002_RS14835	31909..32721	-	813	WP_143589475.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..124030	124030

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-123)

Antitoxin

• SpoVT-AbrB domain (10-55)

Sequences

Toxin

Protein

• Length: 132 a.a.
• Molecular weight: 15034.43 Da
• Isoelectric Point: 6.7114

>T22709 WP_012308571.1 NC_010480:c27563-27168 [Synechococcus sp. PCC 7002]
MFLLDTNICIYIIKEKPSQVLEKFEQIEPHKLGISIITLAELEYGAAKSMNPAKNYQVIEDFITYLDVFNWDRQASHIYG
ELRATLTRQGTPIGILDTQIAAHCLSLNRVLVTNNVREFERVPGLKVKNWV

Nucleotide

• Length: 396 bp

>T22709 NC_010480:c27563-27168 [Synechococcus sp. PCC 7002]
ATGTTCTTGTTGGATACGAATATTTGTATTTACATCATTAAAGAAAAGCCATCTCAAGTTTTAGAAAAGTTTGAACAAAT
TGAGCCCCACAAATTAGGGATCTCGATTATTACTCTAGCGGAATTAGAGTATGGAGCAGCAAAATCAATGAATCCAGCTA
AAAACTATCAGGTTATTGAAGATTTTATTACTTATTTAGATGTCTTTAACTGGGATCGTCAAGCCAGTCATATTTATGGT
GAACTGCGAGCAACTTTAACTCGTCAGGGCACGCCGATCGGTATCTTAGATACTCAAATCGCTGCCCATTGCCTCAGTTT
GAATCGGGTTTTAGTGACGAATAATGTGCGGGAGTTTGAGCGGGTTCCTGGGTTAAAAGTAAAAAATTGGGTTTGA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9415.67 Da
• Isoelectric Point: 6.3382

>AT22709 WP_012308572.1 NC_010480:c27803-27564 [Synechococcus sp. PCC 7002]
MSQPKTAKLFMNGNSQAVRLPREFRFEGKEVFIYKEGDRVILSPKKRSWRDFFENAPCATPDFMETRDDQPPQEREELF

Nucleotide

• Length: 240 bp

>AT22709 NC_010480:c27803-27564 [Synechococcus sp. PCC 7002]
ATGTCTCAACCTAAAACGGCAAAGTTATTTATGAATGGCAATAGTCAGGCGGTACGGTTGCCTCGTGAGTTTCGGTTTGA
GGGGAAGGAAGTCTTTATTTACAAAGAGGGCGATCGCGTAATTTTGTCGCCGAAGAAGCGATCGTGGCGGGATTTTTTTG
AAAATGCTCCTTGTGCCACGCCAGATTTTATGGAAACACGGGATGATCAACCGCCCCAAGAAAGGGAGGAATTATTCTAA

Structures

Toxin

• AlphaFold DB: B1XR29

Antitoxin

• AlphaFold DB: B1XR30