Detailed information of TA system TA227078

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 8465484..8466113
• Replicon: chromosome
• Accession: NZ_CP088285
• Organism: Bradyrhizobium septentrionale strain 1S1

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: HAP48_RS42305
• Protein ID: WP_166215656.1
• Coordinates: 8465484..8465672 (+)
• Length: 63 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: HAP48_RS42310
• Protein ID: WP_166205656.1
• Coordinates: 8465721..8466113 (+)
• Length: 131 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HAP48_RS42285 (HAP48_0042285)	8461478..8462887	+	1410	WP_166205652.1	HD domain-containing protein	-
HAP48_RS42290 (HAP48_0042290)	8462875..8463390	+	516	WP_166205653.1	hypothetical protein	-
HAP48_RS42295 (HAP48_0042295)	8463679..8464119	-	441	WP_166205654.1	hypothetical protein	-
HAP48_RS42300 (HAP48_0042300)	8464458..8465114	+	657	WP_166205655.1	toll/interleukin-1 receptor domain-containing protein	-
HAP48_RS42305 (HAP48_0042305)	8465484..8465672	+	189	WP_166215656.1	type II toxin-antitoxin system HicA family toxin	Toxin
HAP48_RS42310 (HAP48_0042310)	8465721..8466113	+	393	WP_166205656.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
HAP48_RS42315 (HAP48_0042315)	8466809..8467372	+	564	WP_224496819.1	DNA ligase	-
HAP48_RS42320 (HAP48_0042320)	8467495..8468148	-	654	WP_166205657.1	hypothetical protein	-
HAP48_RS42325 (HAP48_0042325)	8468179..8468682	-	504	WP_166205658.1	hypothetical protein	-
HAP48_RS42330 (HAP48_0042330)	8468797..8469321	-	525	WP_166205659.1	hypothetical protein	-
HAP48_RS42335 (HAP48_0042335)	8469321..8470535	-	1215	WP_166205660.1	peptidoglycan-binding protein	-
HAP48_RS42340 (HAP48_0042340)	8470625..8471053	-	429	WP_166205661.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	8452278..8571463	119185

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (4-124)

Sequences

Toxin

Protein

• Length: 63 a.a.
• Molecular weight: 7038.24 Da
• Isoelectric Point: 11.4427

>T227078 WP_166215656.1 NZ_CP088285:8465484-8465672 [Bradyrhizobium septentrionale]
MRSADVIRVLKDDGWKPVAQKGSHIQFKHPTKSGRVTVPHPKRDIPIGTLRSIEKQADLKLR

Nucleotide

• Length: 189 bp

>T227078 NZ_CP122712:c2083047-2082673 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 131 a.a.
• Molecular weight: 13864.61 Da
• Isoelectric Point: 4.2592

>AT227078 WP_166205656.1 NZ_CP088285:8465721-8466113 [Bradyrhizobium septentrionale]
MRHYIALIHKDADSDYGVSFPDLPGVVTAGTNLDDARAMATEALALHLEGMVEDGEAVPEPSSLEEIMADAENRDGVAVL
IPVPATEVKTVRVNVTLPADVLNEIDHYAEQHGFTRSGFLVQAAKKAIAA

Nucleotide

• Length: 393 bp

>AT227078 NZ_CP122712:c2083504-2083136 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA