Detailed information of TA system TA227072

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): rlegTA/AbiEii-AbiEi
• Location: 8397415..8398945
• Replicon: chromosome
• Accession: NZ_CP088280
• Organism: Bradyrhizobium barranii subsp. barranii strain 323S2

Toxin (Protein)

• Gene name: rlegT
• Uniprot ID: -
• Locus tag: G6321_RS44025
• Protein ID: WP_166349196.1
• Coordinates: 8397415..8398335 (-)
• Length: 307 a.a.

Antitoxin (Protein)

• Gene name: rlegA
• Uniprot ID: A0A810CIT3
• Locus tag: G6321_RS44030
• Protein ID: WP_026312796.1
• Coordinates: 8398325..8398945 (-)
• Length: 207 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G6321_RS44010 (G6321_00044010)	8392676..8393908	-	1233	WP_110115977.1	exonuclease SbcCD subunit D	-
G6321_RS44015 (G6321_00044015)	8393917..8396025	-	2109	WP_166349202.1	DUF87 domain-containing protein	-
G6321_RS44020 (G6321_00044020)	8396033..8397337	-	1305	WP_166349199.1	SIR2 family protein	-
G6321_RS44025 (G6321_00044025)	8397415..8398335	-	921	WP_166349196.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
G6321_RS44030 (G6321_00044030)	8398325..8398945	-	621	WP_026312796.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	Antitoxin
G6321_RS44035 (G6321_00044035)	8399159..8399878	-	720	WP_166349193.1	DCL family protein	-
G6321_RS44040 (G6321_00044040)	8399879..8400163	-	285	WP_224516865.1	hypothetical protein	-
G6321_RS44045 (G6321_00044045)	8400209..8401272	+	1064	Protein_8118	IS630-like element ISRj1 family transposase	-
G6321_RS44050 (G6321_00044050)	8401259..8403478	-	2220	WP_224516863.1	AAA family ATPase	-
G6321_RS44055 (G6321_00044055)	8403471..8403698	-	228	WP_131234255.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	8400625..8401272	647

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (38-264)

Antitoxin

• Transcriptional regulator, AbiEi antitoxin (12-56)

Sequences

Toxin

Protein

• Length: 307 a.a.
• Molecular weight: 34085.18 Da
• Isoelectric Point: 5.1178

>T227072 WP_166349196.1 NZ_CP088280:c8398335-8397415 [Bradyrhizobium barranii subsp. barranii]
MAREPRNIGASVRARLLDRARAERSDFQILLTRYALERLLYRLSASPHRDRFILKGAMLFVTWIADPFRPTRDLDLLGHG
DRDTEAIAETFRAICAQPVVDDGVTFDVAALMATPIREELEYGGVRVRTTATIAGARIPIQVDIGFGDAITPAAVEIDYP
TLLDAPTPHLRAYPVETVVAEKFEALVTLGVANSRLKDFYDLWVISRTFELRRAALAEAIQRTFERRGTVLPSVVPVGLT
DEFAETWAAQWRAFLERDRMAAAPDAFAVTVADLRLFLMPLVVGLKEERIWPPSGPWSPGAAIDEA

Nucleotide

• Length: 921 bp

>T227072 NZ_CP122712:c872611-872276 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin

Protein

• Length: 207 a.a.
• Molecular weight: 23385.28 Da
• Isoelectric Point: 10.0691

>AT227072 WP_026312796.1 NZ_CP088280:c8398945-8398325 [Bradyrhizobium barranii subsp. barranii]
MPSTSAQRLRALDLLKTRGMLRLKDFIAEGIGPETLARLVREEAVVRPARGLYQLPDAHLEAAHTLAEAAVLVPKGVVCL
TSALQYHELTLQMPSAVWMAIDRSAWRPKIDYPPIRFVRFTGSALTEGIERHRIENVDVPITDPARTIVDCFRYRTKIGL
DVAMEGLREGLRRRRCKPDQLWHYARKARVWSVMRPYVEAMVSDGA

Nucleotide

• Length: 621 bp

>AT227072 NZ_CP122712:c872859-872611 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A810CIT3