Detailed information of TA system TA227071

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-Phd
• Location: 7590582..7591285
• Replicon: chromosome
• Accession: NZ_CP088280
• Organism: Bradyrhizobium barranii subsp. barranii strain 323S2

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: G6321_RS40340
• Protein ID: WP_135216337.1
• Coordinates: 7590860..7591285 (+)
• Length: 142 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A8T5VAC8
• Locus tag: G6321_RS40335
• Protein ID: WP_028148610.1
• Coordinates: 7590582..7590863 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G6321_RS40315 (G6321_00040315)	7585655..7586629	-	975	WP_166350160.1	sugar ABC transporter permease	-
G6321_RS40320 (G6321_00040320)	7586629..7587714	-	1086	WP_166350158.1	ABC transporter ATP-binding protein	-
G6321_RS40325 (G6321_00040325)	7587729..7588805	-	1077	WP_166350156.1	ABC transporter ATP-binding protein	-
G6321_RS40330 (G6321_00040330)	7588802..7590352	-	1551	WP_166350154.1	glycerol-3-phosphate dehydrogenase	-
G6321_RS40335 (G6321_00040335)	7590582..7590863	+	282	WP_028148610.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
G6321_RS40340 (G6321_00040340)	7590860..7591285	+	426	WP_135216337.1	type II toxin-antitoxin system VapC family toxin	Toxin
G6321_RS40345 (G6321_00040345)	7591290..7592138	-	849	WP_135216336.1	DeoR/GlpR family DNA-binding transcription regulator	-
G6321_RS40350 (G6321_00040350)	7592230..7592928	-	699	WP_166350152.1	HAD-IA family hydrolase	-
G6321_RS40355 (G6321_00040355)	7593083..7593550	+	468	WP_085398540.1	nuclear transport factor 2 family protein	-
G6321_RS40360 (G6321_00040360)	7593878..7594273	+	396	WP_135216334.1	nuclear transport factor 2 family protein	-
G6321_RS40365 (G6321_00040365)	7594592..7594783	-	192	WP_028134810.1	hypothetical protein	-
G6321_RS40370 (G6321_00040370)	7595004..7595414	-	411	WP_063985193.1	acyl-CoA thioesterase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-123)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (14-77)

Sequences

Toxin

Protein

• Length: 142 a.a.
• Molecular weight: 15413.66 Da
• Isoelectric Point: 5.1538

>T227071 WP_135216337.1 NZ_CP088280:7590860-7591285 [Bradyrhizobium barranii subsp. barranii]
VNLLLDTNVLSEVQRPAPSPKVLAWLDTVDEDRAFISVASIAELRRGIALLDDGRRRAALAAWLAHDLPARFAQRILPID
HAVAERWGDLMAQSRRNGVALSVMDGFFAATALANDLTLVTRNVKDFAAFGVPLLDPWSDA

Nucleotide

• Length: 426 bp

>T227071 NZ_CP122712:743980-744357 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGATACTGCTCAC
CCGACTGCTGGACCAGCATTACGGCCTCACGCTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGCGATGCGGTGAACTTTCTCGTGGAAAAATACGCACTGGTACGAACCGACCAGCCGGGGTTC
AGCACCTGTACCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGACCCGCCGGGCAACCGGCCTGATGGCCCGCGA
CAATTACAGAACGGTAAATAACATTATCTTGGGTAAACATCCGGGGGCGAAACAATGA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10250.47 Da
• Isoelectric Point: 5.1409

>AT227071 WP_028148610.1 NZ_CP088280:7590582-7590863 [Bradyrhizobium barranii subsp. barranii]
MTDRSTAPDSPPTDTWTLANAKARLSEVVDRAQTGPQIITRHGRPNAVVVSADEWARKTARKGTLAEFLLASPLRGADLE
LERVQDEPRDEMP

Nucleotide

• Length: 282 bp

>AT227071 NZ_CP122712:743522-743890 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACGCATCCCGACGATAACAACGACCGCCCCTGGTGGGGGCTACCCTGCTCCGTGAC
GCCCTGTTTCGGTGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCAGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATTCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAGCTCATGCTCACCAGCGGT
GAACTCAGCCCCCGCCATCAGCATACCGTGACACTGTATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGTTG
TGGCTACGTTTATATGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA