Detailed information of TA system TA022707

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 96164..96744
• Replicon: plasmid pAQ7
• Accession: NC_010474
• Organism: Synechococcus sp. PCC 7002

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: SYNPCC7002_RS15635
• Protein ID: WP_041444131.1
• Coordinates: 96164..96508 (-)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A1B1U2U2
• Locus tag: SYNPCC7002_RS15640
• Protein ID: WP_030008115.1
• Coordinates: 96502..96744 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SYNPCC7002_RS15615	91406..94054	+	2649	WP_041444130.1	TonB-dependent hemoglobin/transferrin/lactoferrin family receptor	-
SYNPCC7002_RS15620	94180..94545	+	366	WP_041444158.1	VOC family protein	-
SYNPCC7002_RS15625	94610..95497	+	888	WP_143589451.1	ABC transporter substrate-binding protein	-
SYNPCC7002_RS15630	95562..96101	+	540	WP_012305561.1	hypothetical protein	-
SYNPCC7002_RS15635	96164..96508	-	345	WP_041444131.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
SYNPCC7002_RS15640	96502..96744	-	243	WP_030008115.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
SYNPCC7002_RS15645	96849..97664	-	816	WP_012305563.1	ABC transporter ATP-binding protein	-
SYNPCC7002_RS15650	97668..98705	-	1038	WP_041444133.1	iron ABC transporter permease	-
SYNPCC7002_RS15655	98702..99676	-	975	WP_012305565.1	iron ABC transporter permease	-
SYNPCC7002_RS15660	99795..100562	-	768	WP_012305566.1	MORN repeat-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..186459	186459

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (10-110)

Antitoxin

• Antitoxin PemI-like (3-80)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 12861.20 Da
• Isoelectric Point: 9.1547

>T22707 WP_041444131.1 NC_010474:c96508-96164 [Synechococcus sp. PCC 7002]
MVKINGMVPKRGDIIFLELNPRTGSEQSGYRPAIVISPEEYNRISNLTLICPITNKEKGWVFEVKLPPNLKTQGVILVDQ
IRAVDCRARKAKIVEQLPLDTLNEVLARLEPLLI

Nucleotide

• Length: 345 bp

>T22707 NC_010474:c96508-96164 [Synechococcus sp. PCC 7002]
TTGGTAAAAATTAACGGCATGGTTCCTAAGCGGGGGGATATTATTTTTCTCGAACTGAATCCCCGCACAGGTAGTGAACA
ATCAGGTTATCGTCCGGCGATCGTGATTTCTCCTGAGGAATATAACCGTATTTCTAATCTGACCCTAATCTGTCCAATCA
CGAATAAAGAAAAAGGCTGGGTGTTTGAGGTGAAACTTCCGCCTAATCTCAAAACCCAAGGCGTCATCCTGGTCGATCAA
ATCCGGGCGGTGGACTGTCGAGCCAGGAAAGCAAAAATTGTGGAACAACTTCCCCTTGACACTCTTAATGAAGTACTCGC
GAGGTTAGAGCCTCTTTTGATTTAG

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9119.28 Da
• Isoelectric Point: 4.5125

>AT22707 WP_030008115.1 NC_010474:c96744-96502 [Synechococcus sp. PCC 7002]
MITQKITKWGNSFGIRLPQSMLQQCGWQEGEQVTISMENNQIILSLARPKYSLAELLKDAKPEAQHSEVDWGEAQGEESW

Nucleotide

• Length: 243 bp

>AT22707 NC_010474:c96744-96502 [Synechococcus sp. PCC 7002]
ATGATCACCCAAAAAATCACGAAATGGGGCAACTCTTTTGGTATTCGCTTGCCTCAATCCATGCTCCAGCAATGCGGTTG
GCAGGAAGGGGAACAAGTGACGATATCAATGGAAAACAACCAAATTATTTTGAGCCTTGCTCGACCAAAATATAGCCTTG
CGGAATTACTCAAGGATGCAAAACCTGAAGCACAGCATTCTGAAGTGGATTGGGGGGAAGCCCAAGGAGAAGAGTCTTGG
TAA

Structures

Antitoxin

• AlphaFold DB: A0A1B1U2U2