Detailed information of TA system TA227067

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpA-brnA/BrnT-BrnA
• Location: 3246289..3246853
• Replicon: chromosome
• Accession: NZ_CP088273
• Organism: Phyllobacterium sp. T1293

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: -
• Locus tag: LLE53_RS15985
• Protein ID: WP_113095408.1
• Coordinates: 3246578..3246853 (-)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: -
• Locus tag: LLE53_RS15980
• Protein ID: WP_113095407.1
• Coordinates: 3246289..3246585 (-)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LLE53_RS15950 (LLE53_015950)	3241737..3242129	+	393	WP_227987643.1	DUF983 domain-containing protein	-
LLE53_RS15955 (LLE53_015955)	3242179..3242919	-	741	WP_227987644.1	3-oxoacyl-ACP reductase family protein	-
LLE53_RS15960 (LLE53_015960)	3243021..3243626	+	606	WP_227987645.1	TetR/AcrR family transcriptional regulator	-
LLE53_RS15965 (LLE53_015965)	3243683..3244144	-	462	WP_182509251.1	30S ribosomal protein S16	-
LLE53_RS15970 (LLE53_015970)	3244162..3244470	-	309	WP_112522888.1	chorismate mutase	-
LLE53_RS15975 (LLE53_015975)	3244473..3246044	-	1572	WP_227987646.1	signal recognition particle protein	-
LLE53_RS15980 (LLE53_015980)	3246289..3246585	-	297	WP_113095407.1	BrnA antitoxin family protein	Antitoxin
LLE53_RS15985 (LLE53_015985)	3246578..3246853	-	276	WP_113095408.1	BrnT family toxin	Toxin
LLE53_RS15990 (LLE53_015990)	3247188..3248009	+	822	WP_227987647.1	lytic transglycosylase domain-containing protein	-
LLE53_RS15995 (LLE53_015995)	3248067..3248477	-	411	WP_227987648.1	nuclear transport factor 2 family protein	-
LLE53_RS16000 (LLE53_016000)	3248481..3249149	-	669	WP_112522893.1	carbonic anhydrase	-
LLE53_RS16005 (LLE53_016005)	3249320..3250204	-	885	WP_091879563.1	pyridoxal kinase PdxY	-
LLE53_RS16010 (LLE53_016010)	3250254..3250514	-	261	WP_113095553.1	hypothetical protein	-
LLE53_RS16015 (LLE53_016015)	3250662..3251273	+	612	WP_227988232.1	LemA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (6-82)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (24-92)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10852.19 Da
• Isoelectric Point: 7.9911

>T227067 WP_113095408.1 NZ_CP088273:c3246853-3246578 [Phyllobacterium sp. T1293]
MSRFEWNEDKAKSNIRKHGVTFELADRFEFCTAMEQLDDRENYREERTVAIGFIGNVLFTLTYTERGSKIRVISLRKSST
SEIKRYAREYD

Nucleotide

• Length: 276 bp

>T227067 NZ_CP122687:48730-49005 [Escherichia coli]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTGGCCAGTAAACCTGC
TGCCGCCAGAACGGTACAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAACTTAATGACCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 10764.27 Da
• Isoelectric Point: 10.4623

>AT227067 WP_113095407.1 NZ_CP088273:c3246585-3246289 [Phyllobacterium sp. T1293]
MTEKQRAGHRARIQAALDSISPEEDAALTKAALDDPDTVLITELSRRKPGRPLADITKTPVSIRLSPDVVDYFRSSGPGW
QSRIDDVLRKAAGLKKRA

Nucleotide

• Length: 297 bp

>AT227067 NZ_CP122687:48446-48730 [Escherichia coli]
ATGCAAATGAAAAACAATACCGCACAGGCAACAAAAGTAATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGGCTGGAACGCTCCCGGGGCTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA