Detailed information of TA system TA227066

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 2596384..2596955
• Replicon: chromosome
• Accession: NZ_CP088273
• Organism: Phyllobacterium sp. T1293

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: LLE53_RS12780
• Protein ID: WP_227987322.1
• Coordinates: 2596384..2596752 (-)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: LLE53_RS12785
• Protein ID: WP_112523516.1
• Coordinates: 2596749..2596955 (-)
• Length: 69 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LLE53_RS12755 (LLE53_012755)	2591742..2592761	-	1020	WP_227987319.1	PhoH family protein	-
LLE53_RS12760 (LLE53_012760)	2592793..2594232	-	1440	WP_112523499.1	tRNA (N6-isopentenyl adenosine(37)-C2)-methylthiotransferase MiaB	-
LLE53_RS12765 (LLE53_012765)	2594242..2595108	-	867	WP_227988210.1	lysophospholipid acyltransferase family protein	-
LLE53_RS12770 (LLE53_012770)	2595113..2595616	-	504	WP_227987320.1	ribosomal protein S18-alanine N-acetyltransferase	-
LLE53_RS12775 (LLE53_012775)	2595613..2596302	-	690	WP_227987321.1	tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex dimerization subunit type 1 TsaB	-
LLE53_RS12780 (LLE53_012780)	2596384..2596752	-	369	WP_227987322.1	type II toxin-antitoxin system VapC family toxin	Toxin
LLE53_RS12785 (LLE53_012785)	2596749..2596955	-	207	WP_112523516.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
LLE53_RS12790 (LLE53_012790)	2597033..2597875	-	843	WP_227987323.1	DUF2066 domain-containing protein	-
LLE53_RS12795 (LLE53_012795)	2597912..2599954	-	2043	WP_227987324.1	HWE histidine kinase domain-containing protein	-
LLE53_RS12800 (LLE53_012800)	2600082..2600507	-	426	WP_227987325.1	GNAT family N-acetyltransferase	-
LLE53_RS12805 (LLE53_012805)	2600534..2601049	-	516	WP_227987326.1	DUF3859 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	kdsB	2494305..2641284	146979

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13799.89 Da
• Isoelectric Point: 5.9734

>T227066 WP_227987322.1 NZ_CP088273:c2596752-2596384 [Phyllobacterium sp. T1293]
MILADTSVWIDHLRYGDAELVRHLEAAQILIHPFIVGELALGNLRQRELILNSLQDLPKVQVADDKEISYFIDAETIFDL
GLGYVDAHLLAATRLTPGTRLWTRDKRLAAAATRLRLQADFH

Nucleotide

• Length: 369 bp

>T227066 NZ_CP122686:37767-38045 [Escherichia coli]
ATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGACGGC
CGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAGCCTGGCCGGGCAGCCGTATAAAGGTCGCAATGGTCGTG
TGGAAGGTACCCGGGAACTGGTTATTCACCCGCATTTTGTTCTGGTTTATGAGGTCGATAGCCAGTGGGGAAAAGTGTAT
ATTCTGCGAGTGTTGCATACAGCACAGAAGTGGCCATAA

Antitoxin

Protein

• Length: 69 a.a.
• Molecular weight: 7519.66 Da
• Isoelectric Point: 6.2743

>AT227066 WP_112523516.1 NZ_CP088273:c2596955-2596749 [Phyllobacterium sp. T1293]
MRTTLALDDDLVAKAQEYTGLREKSALMRAALKALINIESARRLALLGGSEPDLNTIPRRQITPDVQE

Nucleotide

• Length: 207 bp

>AT227066 NZ_CP122686:37498-37767 [Escherichia coli]
ATGGCACAGGTTAATATGAGTTTAAGAATCGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGGCTCCCAGTTAATCCGGGATTTTATGCGCCAGACCGTTGAACGGCAGCATAATACCTGGTTCCGTGACCAGGTTG
CGGCAGGACGTCAGCAACTCAAGCACGGCGATGTGCTTCCTCATGACATGGTCGAATCCTCCGCTGCTGCATGGCGGGAT
GAAATGAGCAGGAAGGTCGCCGGTAAATGA