Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 52069..52805 Replicon plasmid unnamed1
Accession NZ_CP088270
Organism Klebsiella pneumoniae strain CRKP-27

Toxin (Protein)


Gene name tacT Uniprot ID A0A2J5Q928
Locus tag LQ775_RS26335 Protein ID WP_004098919.1
Coordinates 52323..52805 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID A0A7D3T1D0
Locus tag LQ775_RS26330 Protein ID WP_004213599.1
Coordinates 52069..52335 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LQ775_RS26305 (LQ775_26270) 47301..47858 + 558 WP_004213590.1 recombinase family protein -
LQ775_RS26310 (LQ775_26275) 47861..50830 + 2970 WP_004213592.1 Tn3 family transposase -
LQ775_RS26315 (LQ775_26280) 50872..51366 + 495 WP_004213594.1 hypothetical protein -
LQ775_RS26320 (LQ775_26285) 51427..51630 + 204 WP_004213596.1 HHA domain-containing protein -
LQ775_RS26325 (LQ775_26290) 51644..51874 + 231 WP_004213598.1 hypothetical protein -
LQ775_RS26330 (LQ775_26295) 52069..52335 + 267 WP_004213599.1 DUF1778 domain-containing protein Antitoxin
LQ775_RS26335 (LQ775_26300) 52323..52805 + 483 WP_004098919.1 GNAT family N-acetyltransferase Toxin
LQ775_RS26340 (LQ775_26305) 53226..54453 + 1228 Protein_52 IS3 family transposase -
LQ775_RS26345 (LQ775_26310) 54449..54844 - 396 Protein_53 IS3 family transposase -
LQ775_RS26350 (LQ775_26315) 55019..56041 - 1023 WP_004214536.1 porphobilinogen synthase -
LQ775_RS26355 (LQ775_26320) 56105..57451 - 1347 WP_011154555.1 dihydroorotase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - iroB / iroC / iroD / iroN / rmpA / iucA / iucB / iucC / iucD / iutA / rmpA / rmpA2 1..209636 209636
- inside IScluster/Tn - - 47301..54453 7152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(66-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17335.06 Da        Isoelectric Point: 10.0704

>T227053 WP_004098919.1 NZ_CP088270:52323-52805 [Klebsiella pneumoniae]
VGRITAPEPLSSAHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKKGTKKVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T227053 NZ_CP122685:2464590-2464877 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10151.58 Da        Isoelectric Point: 4.5141

>AT227053 WP_004213599.1 NZ_CP088270:52069-52335 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRVLIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDDSAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT227053 NZ_CP122685:2464351-2464590 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J5Q928

Antitoxin

Source ID Structure
AlphaFold DB A0A7D3T1D0

References