Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5299048..5299673 Replicon chromosome
Accession NZ_CP088269
Organism Klebsiella pneumoniae strain CRKP-27

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag LQ775_RS25715 Protein ID WP_002882817.1
Coordinates 5299048..5299431 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag LQ775_RS25720 Protein ID WP_004150355.1
Coordinates 5299431..5299673 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LQ775_RS25700 (5296414) 5296414..5297316 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
LQ775_RS25705 (5297313) 5297313..5297948 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
LQ775_RS25710 (5297945) 5297945..5298874 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
LQ775_RS25715 (5299048) 5299048..5299431 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
LQ775_RS25720 (5299431) 5299431..5299673 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
LQ775_RS25725 (5299878) 5299878..5300795 + 918 WP_004178029.1 alpha/beta hydrolase -
LQ775_RS25730 (5300809) 5300809..5301750 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
LQ775_RS25735 (5301795) 5301795..5302232 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
LQ775_RS25740 (5302229) 5302229..5303089 - 861 WP_004146232.1 virulence factor BrkB family protein -
LQ775_RS25745 (5303083) 5303083..5303682 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T227052 WP_002882817.1 NZ_CP088269:c5299431-5299048 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T227052 NZ_CP122685:2165532-2165635 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT227052 WP_004150355.1 NZ_CP088269:c5299673-5299431 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT227052 NZ_CP122685:c2165673-2165402 [Escherichia coli]
AAAGTCAGCGAAGGGAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGCGGTGGCCATCAGCTTAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References