Detailed information of TA system TA227051

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mosAT/AbiEii-AbiEi
• Location: 4975078..4976759
• Replicon: chromosome
• Accession: NZ_CP088269
• Organism: Klebsiella pneumoniae strain CRKP-27

Toxin (Protein)

• Gene name: mosT
• Uniprot ID: A0A486TL08
• Locus tag: LQ775_RS24205
• Protein ID: WP_032417722.1
• Coordinates: 4975078..4975995 (-)
• Length: 306 a.a.

Antitoxin (Protein)

• Gene name: mosA
• Uniprot ID: A6TH05
• Locus tag: LQ775_RS24210
• Protein ID: WP_004177809.1
• Coordinates: 4976001..4976759 (-)
• Length: 253 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LQ775_RS24185 (4970384)	4970384..4971904	+	1521	WP_002885173.1	sugar ABC transporter ATP-binding protein	-
LQ775_RS24190 (4971901)	4971901..4972899	+	999	WP_002885172.1	ribose ABC transporter permease	-
LQ775_RS24195 (4972930)	4972930..4973871	+	942	WP_032417723.1	ABC transporter substrate-binding protein	-
LQ775_RS24200 (4973946)	4973946..4974953	+	1008	WP_004178300.1	carbohydrate kinase family protein	-
LQ775_RS24205 (4975078)	4975078..4975995	-	918	WP_032417722.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
LQ775_RS24210 (4976001)	4976001..4976759	-	759	WP_004177809.1	type IV toxin-antitoxin system AbiEi family antitoxin	Antitoxin
LQ775_RS24215 (4976845)	4976845..4977573	-	729	WP_004177810.1	response regulator transcription factor	-
LQ775_RS24220 (4977846)	4977846..4979993	+	2148	WP_077598858.1	formate dehydrogenase subunit alpha	-
LQ775_RS24225 (4979990)	4979990..4980133	-	144	WP_165443020.1	hypothetical protein	-
LQ775_RS24230 (4980339)	4980339..4981025	+	687	WP_004151733.1	tetratricopeptide repeat protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (14-278)

Antitoxin

• Transcriptional regulator AbiEi antitoxin, N-terminal domain (1-93)

Sequences

Toxin

Protein

• Length: 306 a.a.
• Molecular weight: 34674.07 Da
• Isoelectric Point: 5.4707

>T227051 WP_032417722.1 NZ_CP088269:c4975995-4975078 [Klebsiella pneumoniae]
MDIQAPYYRQVALLMQVLPYVAVEREFALKGGTAINLFIRDFPRLSVDIDLAWVPQESRAIALPHIRDALARIAANLQQQ
AGMSAVLQANRSDEMRVIVTTDSAQIKIEVSPVARGTLYPPQEREVVGAVEDVFGYASLPVVSLPDLYGGKLCAALDRQH
PRDFYDVKLLLDAQELDRPIFNGFIAYLLSHNRPLAEVLNPRWKDIAEPFYREFSGMTFETIALEELTAVPNRMIAALKS
CFTQQDVDFLLSFKRGEPDWRLAPEMRIQDLPAVQWKLRNIHQMPAIKRAESLDKLEKVLAEWRS

Nucleotide

• Length: 918 bp

>T227051 NZ_CP122685:1967254-1967508 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAGTCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 253 a.a.
• Molecular weight: 28548.92 Da
• Isoelectric Point: 10.0870

>AT227051 WP_004177809.1 NZ_CP088269:c4976759-4976001 [Klebsiella pneumoniae]
MATKLNWLLQNSAPGDVILQSWLSQHAISPSLAFKYAQSGWLKKRGNGVYARAGREPEWNDALACLQNQLAAPVYVAGLS
SLVWQGRSHYLQLKQNQCWLSVENKALLPKWFREFPGVEWIVISGKKLPVLDDRYRVTLDIKGKKLIGSAPELAAYELLS
AVPGTLSFNHAAELFQGLVNLNPRKVEYLLSVSQSVQAKRLYLFFASFYEHGWLKRIDSQKIDLGAGKRQIVENGKFNAQ
YQITVPERFQKE

Nucleotide

• Length: 759 bp

>AT227051 NZ_CP122685:1967006-1967257 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCTGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Structures

Toxin

• AlphaFold DB: A0A486TL08

Antitoxin

• AlphaFold DB: A6TH05