Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) chpSB/PRK09812-ChpS
Location 4546832..4547427 Replicon chromosome
Accession NC_010473
Organism Escherichia coli str. K-12 substr. DH10B

Toxin (Protein)


Gene name chpB Uniprot ID U9XNP6
Locus tag ECDH10B_RS22550 Protein ID WP_000239577.1
Coordinates 4547077..4547427 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name chpS Uniprot ID U9Y2K1
Locus tag ECDH10B_RS22545 Protein ID WP_001223208.1
Coordinates 4546832..4547083 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECDH10B_RS22535 (ECDH10B_4416) 4542497..4546276 + 3780 WP_000060911.1 autotransporter assembly complex protein TamB -
ECDH10B_RS22540 (ECDH10B_4417) 4546279..4546620 + 342 WP_001219160.1 gamma-glutamylcyclotransferase -
ECDH10B_RS22545 (ECDH10B_4419) 4546832..4547083 + 252 WP_001223208.1 type II toxin-antitoxin system antitoxin ChpS Antitoxin
ECDH10B_RS22550 (ECDH10B_4420) 4547077..4547427 + 351 WP_000239577.1 endoribonuclease toxin ChpB Toxin
ECDH10B_RS22555 (ECDH10B_4421) 4547507..4548037 - 531 WP_000055075.1 inorganic diphosphatase -
ECDH10B_RS22560 (ECDH10B_4422) 4548347..4549303 + 957 WP_000265913.1 galactofuranose ABC transporter substrate-binding protein YtfQ -
ECDH10B_RS22565 (ECDH10B_4423) 4549443..4550945 + 1503 WP_000205805.1 sugar ABC transporter ATP-binding protein -
ECDH10B_RS25780 4550959..4551090 + 132 Protein_4277 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-114)

Antitoxin

SpoVT-AbrB domain

(6-49)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12492.40 Da        Isoelectric Point: 5.5572

>T22705 WP_000239577.1 NC_010473:4547077-4547427 [Escherichia coli str. K-12 substr. DH10B]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLHARLAKRIGLAADEVVEEALLRLQAVVE

Download         Length: 351 bp

>T22705 NC_010473:4547077-4547427 [Escherichia coli str. K-12 substr. DH10B]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACACGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9271.58 Da        Isoelectric Point: 4.4201

>AT22705 WP_001223208.1 NC_010473:4546832-4547083 [Escherichia coli str. K-12 substr. DH10B]
MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGD
EIW

Download         Length: 252 bp

>AT22705 NC_010473:4546832-4547083 [Escherichia coli str. K-12 substr. DH10B]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XNP6

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LQ26

References