Detailed information of TA system TA227034

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazF-pemI/MazF(toxin)
• Location: 732880..733516
• Replicon: chromosome
• Accession: NZ_CP088268
• Organism: Metabacillus litoralis strain NCTR108

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: A0A0M2PN83
• Locus tag: KDJ21_RS03655
• Protein ID: WP_026562078.1
• Coordinates: 732880..733230 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: -
• Locus tag: KDJ21_RS03660
• Protein ID: WP_098797598.1
• Coordinates: 733235..733516 (-)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KDJ21_RS03620 (KDJ21_003620)	728279..729070	-	792	WP_212138423.1	RNA polymerase sigma factor SigB	-
KDJ21_RS03625 (KDJ21_003625)	729036..729518	-	483	WP_121664246.1	anti-sigma B factor RsbW	-
KDJ21_RS03630 (KDJ21_003630)	729515..729847	-	333	WP_212138424.1	anti-sigma factor antagonist	-
KDJ21_RS03635 (KDJ21_003635)	729903..730910	-	1008	WP_212138425.1	PP2C family protein-serine/threonine phosphatase	-
KDJ21_RS03640 (KDJ21_003640)	730921..731321	-	401	Protein_665	anti-sigma regulatory factor	-
KDJ21_RS03645 (KDJ21_003645)	731325..731687	-	363	WP_098797600.1	STAS domain-containing protein	-
KDJ21_RS03650 (KDJ21_003650)	731691..732521	-	831	WP_212138427.1	STAS domain-containing protein	-
KDJ21_RS03655 (KDJ21_003655)	732880..733230	-	351	WP_026562078.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
KDJ21_RS03660 (KDJ21_003660)	733235..733516	-	282	WP_098797598.1	antitoxin endoai	Antitoxin
KDJ21_RS03665 (KDJ21_003665)	733843..734986	-	1144	Protein_670	alanine racemase	-
KDJ21_RS03670 (KDJ21_003670)	735264..736292	-	1029	WP_232273699.1	outer membrane lipoprotein carrier protein LolA	-
KDJ21_RS03675 (KDJ21_003675)	736499..736864	-	366	WP_212138430.1	holo-ACP synthase	-
KDJ21_RS03680 (KDJ21_003680)	736996..737715	+	720	WP_212138431.1	rhomboid family intramembrane serine protease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13020.06 Da
• Isoelectric Point: 4.8781

>T227034 WP_026562078.1 NZ_CP088268:c733230-732880 [Metabacillus litoralis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLIIQNDIGNRFSPTVIIAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLGLIDF

Nucleotide

• Length: 351 bp

>T227034 NZ_CP122681:2149212-2149315 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10598.08 Da
• Isoelectric Point: 5.8851

>AT227034 WP_098797598.1 NZ_CP088268:c733516-733235 [Metabacillus litoralis]
VSESSATTEILIQLPQALVSELDVLVKQENGNRNELIYQATKMYIRERKKRQIRESMRRGYMEMAKINLNIASEAFLAES
EADHTVERLVSGG

Nucleotide

• Length: 282 bp

>AT227034 NZ_CP122681:c2149353-2149082 [Escherichia coli]
AAAGTCAGCGAAGGGAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGCGGTGGCCATCAGCTTAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Structures

Toxin

• AlphaFold DB: A0A0M2PN83