Detailed information of TA system TA227033

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 3697372..3698946
• Replicon: chromosome
• Accession: NZ_CP088265
• Organism: Leclercia sp. G3L

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: LRS40_RS19135
• Protein ID: WP_231312381.1
• Coordinates: 3697372..3698691 (-)
• Length: 440 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: LRS40_RS19140
• Protein ID: WP_142489448.1
• Coordinates: 3698695..3698946 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LRS40_RS19120 (LRS40_19120)	3692897..3694078	-	1182	WP_142489444.1	PLP-dependent aminotransferase family protein	-
LRS40_RS19125 (LRS40_19125)	3694178..3696172	-	1995	WP_231312379.1	TonB-dependent receptor	-
LRS40_RS19130 (LRS40_19130)	3696183..3697244	-	1062	WP_231312380.1	YncE family protein	-
LRS40_RS19135 (LRS40_19135)	3697372..3698691	-	1320	WP_231312381.1	type II toxin-antitoxin system HipA family toxin	Toxin
LRS40_RS19140 (LRS40_19140)	3698695..3698946	-	252	WP_142489448.1	helix-turn-helix domain-containing protein	Antitoxin
LRS40_RS19145 (LRS40_19145)	3699751..3703551	+	3801	WP_231312382.1	glycosyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (6-105)

Antitoxin

• Cro/C1-type helix-turn-helix domain (10-64)

Sequences

Toxin

Protein

• Length: 440 a.a.
• Molecular weight: 48588.98 Da
• Isoelectric Point: 6.9657

>T227033 WP_231312381.1 NZ_CP088265:c3698691-3697372 [Leclercia sp. G3L]
MRAKRLTVAMNGDVIGTLHRDGSGAMSFQYAPAWLAEPGSRAISLSLPLQHGRISGAPVFNFFSNLLPDSEAIIARMQAR
FQVETTHPFDLLASVGRDCVGAIQLYPPGQEIPPVTDIVAEPLDDDQIAALLEGYQMAPLGMTEEGADFRISLAGAQEKT
ALLWYQDRWQRPQGSTPTSHIFKLPIGRIEQNNIDLSESCENEWLCLRIAKAFGFPVAHADMAQFGRKKVLIIERFDRRW
ARSGWLMRLPQEDFCQALGVSPALKYESHGGPGIADAMKLLLGSRLAGQDREMFFKAQILFWMLAAIDGHGKNFSLFIEA
DSSFRMAPLYDVISAFPLFDAGGIPVKKAKMAMALQGKNRQYHFAMIQPRHFISTAAFAGFSQEAARRLMTEMAAVTDDV
IATVRAELPTDFPLPVSEAIFRGLARQAARIGRFNELTP

Nucleotide

• Length: 1320 bp

>T227033 NZ_CP122681:1947670-1947924 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAGTCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9166.46 Da
• Isoelectric Point: 9.8953

>AT227033 WP_142489448.1 NZ_CP088265:c3698946-3698695 [Leclercia sp. G3L]
MKVTSADALAQAVRDQRKVNKLTQTATAEQVGIKQTTVSDFENKPGSTKLDTLFKILAALDLELHVVKRGATLPDNNAWT
KEW

Nucleotide

• Length: 252 bp

>AT227033 NZ_CP122681:1947422-1947673 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCTGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA