Detailed information of TA system TA227023

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-MazE
• Location: 2906279..2906869
• Replicon: chromosome
• Accession: NZ_CP088265
• Organism: Leclercia sp. G3L

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: -
• Locus tag: LRS40_RS15195
• Protein ID: WP_142486330.1
• Coordinates: 2906537..2906869 (+)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: -
• Locus tag: LRS40_RS15190
• Protein ID: WP_231311958.1
• Coordinates: 2906279..2906536 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LRS40_RS15170 (LRS40_15170)	2902772..2903728	+	957	WP_231311957.1	L,D-transpeptidase	-
LRS40_RS15180 (LRS40_15180)	2904181..2905098	+	918	WP_142486333.1	nitrogen assimilation transcriptional regulator NAC	-
LRS40_RS15185 (LRS40_15185)	2905195..2906145	+	951	WP_142486332.1	HTH-type transcriptional regulator Cbl	-
LRS40_RS15190 (LRS40_15190)	2906279..2906536	+	258	WP_231311958.1	antitoxin	Antitoxin
LRS40_RS15195 (LRS40_15195)	2906537..2906869	+	333	WP_142486330.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
LRS40_RS15205 (LRS40_15205)	2907115..2907912	-	798	WP_156264165.1	DgsA anti-repressor MtfA	-
LRS40_RS15215 (LRS40_15215)	2908259..2908984	-	726	WP_156264164.1	two-component system response regulator DcuR	-
LRS40_RS15220 (LRS40_15220)	2908981..2910594	-	1614	WP_231311959.1	sensor histidine kinase	-
LRS40_RS15225 (LRS40_15225)	2910728..2911801	-	1074	WP_231311960.1	porin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-108)

Antitoxin

• Antitoxin PemI-like (5-85)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11813.77 Da
• Isoelectric Point: 10.4752

>T227023 WP_142486330.1 NZ_CP088265:2906537-2906869 [Leclercia sp. G3L]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKAAFNHLTRLPVVVPVTTGGKFARTAGFAVSLDEAGSKTRGVIRCDQPRTI
DMGARHGIKLERVPDSVMNEVLAKLGALLT

Nucleotide

• Length: 333 bp

>T227023 NZ_CP122679:c4318911-4318624 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGAAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTATTGTTTGATGATCCACAACATTTGTCACAGCAAGAACGCATAGAAAACGGTGAGTATCGCTGGCAGACAATCGGCC
TGGTTTACGGCATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9281.72 Da
• Isoelectric Point: 4.2941

>AT227023 WP_231311958.1 NZ_CP088265:2906279-2906536 [Leclercia sp. G3L]
MYITRLKKVGGSVMLAVPPAILKTLDLQPDSEVSVTIDNGCLIVEPQKLPHYSLAELLAQCDPHAELSEEDREWVDAPAA
GKEIL

Nucleotide

• Length: 258 bp

>AT227023 NZ_CP122679:c4318637-4318335 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCGCTGACGAA
TAAATCCGACGAAGATATTGATTACAGTGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCCGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA