Detailed information of TA system TA227020

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-RelB
• Location: 1474777..1475363
• Replicon: chromosome
• Accession: NZ_CP088265
• Organism: Leclercia sp. G3L

Toxin (Protein)

• Gene name: doc
• Uniprot ID: -
• Locus tag: LRS40_RS08390
• Protein ID: WP_142487420.1
• Coordinates: 1474995..1475363 (+)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: -
• Locus tag: LRS40_RS08385
• Protein ID: WP_142487421.1
• Coordinates: 1474777..1474998 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LRS40_RS08360 (LRS40_08360)	1469790..1470899	+	1110	WP_231313504.1	high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK	-
LRS40_RS08365 (LRS40_08365)	1470950..1471876	+	927	WP_039032510.1	high-affinity branched-chain amino acid ABC transporter permease LivH	-
LRS40_RS08370 (LRS40_08370)	1471873..1473150	+	1278	WP_231313505.1	high-affinity branched-chain amino acid ABC transporter permease LivM	-
LRS40_RS08375 (LRS40_08375)	1473147..1473914	+	768	WP_142487422.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG	-
LRS40_RS08380 (LRS40_08380)	1473916..1474629	+	714	WP_156264803.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF	-
LRS40_RS08385 (LRS40_08385)	1474777..1474998	+	222	WP_142487421.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
LRS40_RS08390 (LRS40_08390)	1474995..1475363	+	369	WP_142487420.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
LRS40_RS08395 (LRS40_08395)	1475516..1476832	+	1317	WP_231313506.1	sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB	-
LRS40_RS08400 (LRS40_08400)	1476933..1477820	+	888	WP_142487418.1	sn-glycerol-3-phosphate ABC transporter permease UgpA	-
LRS40_RS08405 (LRS40_08405)	1477817..1478662	+	846	WP_142487417.1	sn-glycerol-3-phosphate ABC transporter permease UgpE	-
LRS40_RS08410 (LRS40_08410)	1478664..1479734	+	1071	WP_231313507.1	sn-glycerol-3-phosphate import ATP-binding protein UgpC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1464669..1480471	15802

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Fido domain (29-86)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-50)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13645.94 Da
• Isoelectric Point: 8.4948

>T227020 WP_142487420.1 NZ_CP088265:1474995-1475363 [Leclercia sp. G3L]
MTLQFISAEEIVLFHDKLLSVTPGVAGMSDPGRAEALLYRVLNKYQYEGVTDVWILAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGISLKANPDYVAMTVEAAAGRLSLEEIVERLRR

Nucleotide

• Length: 369 bp

>T227020 NZ_CP122679:3740359-3740577 [Escherichia coli]
ATGTCCGAAAAACCTTTAACAAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8078.10 Da
• Isoelectric Point: 5.6579

>AT227020 WP_142487421.1 NZ_CP088265:1474777-1474998 [Leclercia sp. G3L]
MRTMNYSEARQNLASALDSAATGTPVTITRRGHKPAVIISAEEFERYQVAKLDAEFDAIMGIHGNEIRELADK

Nucleotide

• Length: 222 bp

>AT227020 NZ_CP122679:3739959-3740333 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG