Detailed information of TA system TA227018

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-MazE
• Location: 1088634..1089296
• Replicon: chromosome
• Accession: NZ_CP088265
• Organism: Leclercia sp. G3L

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: LRS40_RS06535
• Protein ID: WP_197090550.1
• Coordinates: 1088634..1089032 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: LRS40_RS06540
• Protein ID: WP_142490039.1
• Coordinates: 1089036..1089296 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LRS40_RS06510 (LRS40_06510)	1083709..1084797	+	1089	WP_142487702.1	AAA family ATPase	-
LRS40_RS06515 (LRS40_06515)	1084826..1085779	-	954	WP_231313361.1	helix-turn-helix domain-containing protein	-
LRS40_RS06520 (LRS40_06520)	1085806..1086585	-	780	WP_231313362.1	MBL fold metallo-hydrolase	-
LRS40_RS06525 (LRS40_06525)	1086724..1087527	-	804	WP_142487699.1	lipoprotein NlpA	-
LRS40_RS06530 (LRS40_06530)	1087868..1088374	+	507	WP_142487698.1	hypothetical protein	-
LRS40_RS06535 (LRS40_06535)	1088634..1089032	-	399	WP_197090550.1	type II toxin-antitoxin system VapC family toxin	Toxin
LRS40_RS06540 (LRS40_06540)	1089036..1089296	-	261	WP_142490039.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
LRS40_RS06545 (LRS40_06545)	1089414..1090913	-	1500	WP_231313363.1	MDR family MFS transporter	-
LRS40_RS06550 (LRS40_06550)	1090992..1091651	+	660	WP_156262610.1	TetR/AcrR family transcriptional regulator	-
LRS40_RS06555 (LRS40_06555)	1091648..1092067	-	420	WP_142487695.1	GNAT family N-acetyltransferase	-
LRS40_RS06560 (LRS40_06560)	1092096..1092359	-	264	WP_231313364.1	hypothetical protein	-
LRS40_RS06565 (LRS40_06565)	1092389..1092685	+	297	WP_032615642.1	YicS family protein	-
LRS40_RS06570 (LRS40_06570)	1092723..1093670	-	948	WP_142487693.1	LacI family DNA-binding transcriptional regulator	-
LRS40_RS06575 (LRS40_06575)	1093965..1094279	+	315	WP_032615638.1	PTS sugar transporter subunit IIB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1073170..1089296	16126

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-121)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14730.88 Da
• Isoelectric Point: 5.9000

>T227018 WP_197090550.1 NZ_CP088265:c1089032-1088634 [Leclercia sp. G3L]
MLHMLDTNIVSHLVKQHPSVVDRYSQIAPEEMCISSITEAELLYGVAKKQSHRLHETISEFLKTITVCDWDSDAAAIYGK
LRASMEKRGKVMGDLDQLIAAHALSRGTTIVTNDRAFAMVQELTIEDWTTAA

Nucleotide

• Length: 399 bp

>T227018 NZ_CP122679:c3054166-3053789 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTACGGCCTCACACTGAATAACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCACTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCACTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCCCGCCGGGCGACAGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAACATCCGGGGGCGAAACAATGA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 10195.57 Da
• Isoelectric Point: 9.8080

>AT227018 WP_142490039.1 NZ_CP088265:c1089296-1089036 [Leclercia sp. G3L]
MERKAKLFKKGRNQAVLLPAEFAFDSESVWIRRDEEGNVVLRAMSEKERHRENFLRLLKQTQVPDSFLSKEERNQSYTTR
DPLEGL

Nucleotide

• Length: 261 bp

>AT227018 NZ_CP122679:c3054623-3054255 [Escherichia coli]
GTGTCAGACACACTCTCCGGGACAACGCATCCCGATGACAATCATGACCGTCCCTGGTGGGGACTACCCTGCACCGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGCAATCAGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCGTACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAGCTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCCAGAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCAAAATGAAAAATTAA