Detailed information of TA system TA227015

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yfjZ-ypjF/CbtA-CbeA
• Location: 201706..202364
• Replicon: chromosome
• Accession: NZ_CP088265
• Organism: Leclercia sp. G3L

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: -
• Locus tag: LRS40_RS02410
• Protein ID: WP_231313015.1
• Coordinates: 202044..202364 (+)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: A0A851GDW8
• Locus tag: LRS40_RS02405
• Protein ID: WP_045333995.1
• Coordinates: 201706..202023 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LRS40_RS02370 (LRS40_02370)	197567..198271	+	705	WP_231313012.1	transcriptional regulator	-
LRS40_RS02375 (LRS40_02375)	198268..198882	+	615	WP_178328625.1	hypothetical protein	-
LRS40_RS02385 (LRS40_02385)	200184..200411	+	228	WP_231313014.1	DUF905 family protein	-
LRS40_RS02390 (LRS40_02390)	200507..200965	+	459	WP_178328622.1	antirestriction protein	-
LRS40_RS02395 (LRS40_02395)	200981..201457	+	477	WP_045333998.1	RadC family protein	-
LRS40_RS02400 (LRS40_02400)	201466..201687	+	222	WP_021520981.1	DUF987 domain-containing protein	-
LRS40_RS02405 (LRS40_02405)	201706..202023	+	318	WP_045333995.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
LRS40_RS02410 (LRS40_02410)	202044..202364	+	321	WP_231313015.1	TA system toxin CbtA family protein	Toxin
LRS40_RS02415 (LRS40_02415)	202662..203519	-	858	WP_001581455.1	DeoR/GlpR family DNA-binding transcription regulator	-
LRS40_RS02420 (LRS40_02420)	203650..204600	-	951	WP_001581454.1	D-ribose ABC transporter substrate-binding protein	-
LRS40_RS02425 (LRS40_02425)	204689..205750	-	1062	WP_001581452.1	ABC transporter permease	-
LRS40_RS02430 (LRS40_02430)	205774..207312	-	1539	WP_032637388.1	sugar ABC transporter ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-102)

Antitoxin

• Antitoxin CbeA (5-104)

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 12158.19 Da
• Isoelectric Point: 7.1656

>T227015 WP_231313015.1 NZ_CP088265:202044-202364 [Leclercia sp. G3L]
MKTLPATTLWAAKPCPSPVIVWQMLLSRLLDQHYGLTLNDTPFSDETVIKEHIDAGISLADAVNFLVEKYELVRIDRKGF
SWQEQSPFITAVDILKARRAMREMRI

Nucleotide

• Length: 321 bp

>T227015 NZ_CP122679:c2599931-2599755 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTGGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCAAGGGCGACGCAGTGTCATGCCGCGTCACCCCAATGATGAGATAAAAGAACCATTGCGTAAAGCAATCCTGAAGC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 12094.70 Da
• Isoelectric Point: 6.9467

>AT227015 WP_045333995.1 NZ_CP088265:201706-202023 [Leclercia sp. G3L]
MRNITWGLQRDITPRLGARLVQEGNRLHYLADRASITGKFSDVECRKLDETFPHFISQMESMLTTDELNPRHAHCVTLYH
NGFTCEADTLGSCGYIYITVYPTQR

Nucleotide

• Length: 318 bp

>AT227015 NZ_CP122679:c2599709-2599293 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTGCCTTTGAGCGTCGCCTCTAAAGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAAAT
TACTCGCTTATTTAACTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTTGCGGCTAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Antitoxin

• AlphaFold DB: A0A851GDW8