Detailed information of TA system TA227014

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 61654..62273
• Replicon: chromosome
• Accession: NZ_CP088265
• Organism: Leclercia sp. G3L

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: A0A345CDT0
• Locus tag: LRS40_RS01720
• Protein ID: WP_032616864.1
• Coordinates: 62055..62273 (+)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: -
• Locus tag: LRS40_RS01715
• Protein ID: WP_231312958.1
• Coordinates: 61654..62046 (+)
• Length: 131 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LRS40_RS01705 (LRS40_01705)	56805..57998	+	1194	WP_142488465.1	multidrug efflux RND transporter periplasmic adaptor subunit AcrA	-
LRS40_RS01710 (LRS40_01710)	58021..61161	+	3141	WP_231312957.1	multidrug efflux RND transporter permease subunit AcrB	-
LRS40_RS01715 (LRS40_01715)	61654..62046	+	393	WP_231312958.1	Hha toxicity modulator TomB	Antitoxin
LRS40_RS01720 (LRS40_01720)	62055..62273	+	219	WP_032616864.1	HHA domain-containing protein	Toxin
LRS40_RS01725 (LRS40_01725)	62548..63012	+	465	WP_142488462.1	YlaC family protein	-
LRS40_RS01730 (LRS40_01730)	63072..63212	-	141	WP_142488461.1	type B 50S ribosomal protein L36	-
LRS40_RS01735 (LRS40_01735)	63406..64974	+	1569	WP_231312959.1	EAL domain-containing protein	-
LRS40_RS01740 (LRS40_01740)	65007..65360	-	354	WP_142488459.1	DUF1428 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-115)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8583.95 Da
• Isoelectric Point: 8.9008

>T227014 WP_032616864.1 NZ_CP088265:62055-62273 [Leclercia sp. G3L]
MSDKPLTKTDYLMRLRRCQSIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTAVWKFVR

Nucleotide

• Length: 219 bp

>T227014 NZ_CP122679:2557340-2558662 [Escherichia coli]
ATGCCTAAACTTGTCACTTGGATGAACAACCAGCGGGTAGGCGAGTTAACGAAGTTAGCCAATGGCGCGCACACCTTTAA
GTATGCACCGGAGTGGTTAGCCAACCGTTATGCCAGACCGTTATCACTTTCGCTGCCATTGCAGAGGGGGAATATCACCT
CTGATGCTGTATTTAACTTCTTCGATAACCTGCTACCCGATAGCCCGATTGTACGTGACCGGATCGTTAAACGTTATCAT
GCTAAATCCAGACAACCGTTTGATTTATTGTCAGAAATAGGGCGAGATAGCGTTGGTGCCGTAACGTTATTACCCGAAAA
TGAAACCATAACGCGCCCGATAATGGCATGGGAACAGCTCACTGAAGCCAGGCTTGAAGAAGTATTAACGGCTTATAAAG
CAGATATCCCTCTGGGCATGATTAGAGAAGAAAATGACTTTCGCATCTCGGTTGCTGGCGCGCAGGAGAAGACAGCGCTG
CTCAGAATAGGCAATGACTGGTGCATTCCGAAAGGAATAACGCCGACGACGCACATCATTAAATTACCGATTGGCGAAAT
CAGGCAGCCCAATGCGACGCTCGATCTCAGCCAAAGCGTTGATAATGAGTATTACTGTCTGCTGCTGGCGAAAGAACTTG
GGTTGAATGTGCCGGACGCAGAAATCATTAAAGCGGGAAATGTGCGCGCGTTAGCGGTCGAACGTTTTGACAGGCGTTGG
AATGCTGAGCGAACGGTTTTACTTCGCTTGCCACAGGAGGATATGTGTCAGACATTCGGTTTACCTTCATCGGTGAAATA
TGAATCAGATGGAGGCCCAGGCATCGCGCGGATCATGGCTTTTTTGATGGGGTCCAGCGAGGCGCTGAAAGATCGCTATG
ATTTTATGAAGTTTCAGGTGTTCCAGTGGTTGATTGGCGCAACGGATGGCCATGCAAAAAACTTCTCCGTATTTATTCAG
GCTGGCGGCAGTTATCGACTCACGCCATTTTACGACATCATTTCAGCATTTCCGGTATTGGGTGGCGCAGGAATACACAT
CAGCGATCTCAAACTGGCAATGGGGCTTAACGCATCCAAAGGCAAAAAAACGGCAATCGATAAAATTTATCCGCGACATT
TTCTGGCGACAGCAAAGGTGCTGAAATTTCCGGAAGTGCAGATGCATGAAATCCTGAGTGACTTTGCCAGAATGATTCCG
GCAGCACTGGATCATGTGAAGACTTCATTACCGACAGATTTTCCGGAGAACGTGGTGACGGCAGTTGAAACCAATGTGTT
GAGGTTGCATGGACGGTTAAGCCGAGAATACGGTAGTAAGTGA

Antitoxin

Protein

• Length: 131 a.a.
• Molecular weight: 15049.93 Da
• Isoelectric Point: 5.1494

>AT227014 WP_231312958.1 NZ_CP088265:61654-62046 [Leclercia sp. G3L]
MDEYSPKRQDIAQLKFLCESLYHDCLSNLDESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDGYL
DDTFMLFSSYGINAQDLQKWRKSGNRLFRCFVNASRANPLASPVKIITNN

Nucleotide

• Length: 393 bp

>AT227014 NZ_CP122679:2557074-2557340 [Escherichia coli]
ATGATGAGCTTTCAGAAGATCTATAGCCCAACGCAATTAGCGAATGCAATGAAACTGGTTCGTCAGCAAAATGGCTGGAC
GCAGAGCGAGCTGGCGAAAAAAATCGGCATTAAGCAGGCGACAATTTCCAATTTCGAAAACAACCCTGACAATACCACGC
TGACGACATTTTTTAAGATTTTACAGTCGCTTGAACTCTCAATGACGCTATGCGACACGAAAAATGCCTCGCCAGAATCA
ACAGAACAGCAAGATCTGGAGTGGTAA

Structures

Toxin

• AlphaFold DB: A0A345CDT0