Detailed information of TA system TA227013

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 81254..81891
• Replicon: plasmid pG3L-1
• Accession: NZ_CP088263
• Organism: Leclercia sp. G3L

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: LRS40_RS00440
• Protein ID: WP_231311750.1
• Coordinates: 81254..81664 (-)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: -
• Locus tag: LRS40_RS00445
• Protein ID: WP_231311751.1
• Coordinates: 81661..81891 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LRS40_RS00425 (LRS40_00425)	76898..78325	-	1428	WP_231311747.1	phosphotransferase	-
LRS40_RS00430 (LRS40_00430)	78306..79232	-	927	WP_231311748.1	hypothetical protein	-
LRS40_RS00435 (LRS40_00435)	79229..81067	-	1839	WP_231311749.1	AAA family ATPase	-
LRS40_RS00440 (LRS40_00440)	81254..81664	-	411	WP_231311750.1	type II toxin-antitoxin system VapC family toxin	Toxin
LRS40_RS00445 (LRS40_00445)	81661..81891	-	231	WP_231311751.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
LRS40_RS00450 (LRS40_00450)	82493..82843	+	351	WP_000493378.1	hypothetical protein	-
LRS40_RS00455 (LRS40_00455)	82894..83637	+	744	WP_000129823.1	hypothetical protein	-
LRS40_RS00460 (LRS40_00460)	83634..84410	+	777	WP_000015958.1	site-specific integrase	-
LRS40_RS00465 (LRS40_00465)	84468..84725	-	258	WP_000764642.1	hypothetical protein	-
LRS40_RS00470 (LRS40_00470)	84854..84958	-	105	WP_032409716.1	hypothetical protein	-
LRS40_RS00475 (LRS40_00475)	85488..86354	+	867	WP_004118283.1	replication initiation protein	-
LRS40_RS00480 (LRS40_00480)	86531..86800	-	270	WP_000339857.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	ant(3'')-Ia / blaTEM-1B / qnrS1 / floR / blaLAP-2 / tet(A) / dfrA1 / qacE / sul1 / tet(X4) / lnu(G)	-	1..253593	253593

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-127)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 14146.38 Da
• Isoelectric Point: 4.8484

>T227013 WP_231311750.1 NZ_CP088263:c81664-81254 [Leclercia sp. G3L]
VKKTYMLDTGICALILREQPAALLKRLEQAVLDGDRIVVSAITWAEISLAARASGPATQQLADAFAARLDAILPWDRAAV
DATTDVKVALRLAGTPIGLNDTAIAGHAIAAGAVLVTGKGGEFERVPGLTLEECLS

Nucleotide

• Length: 411 bp

>T227013 NZ_CP122679:2495896-2496183 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8769.89 Da
• Isoelectric Point: 4.3775

>AT227013 WP_231311751.1 NZ_CP088263:c81891-81661 [Leclercia sp. G3L]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQMEKADPDFMAEREDVISDEGRFEL

Nucleotide

• Length: 231 bp

>AT227013 NZ_CP122679:2495657-2495896 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA