Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 75706..76349 Replicon plasmid pG3L-1
Accession NZ_CP088263
Organism Leclercia sp. G3L

Toxin (Protein)


Gene name vagD Uniprot ID Q84A06
Locus tag LRS40_RS00415 Protein ID WP_000754566.1
Coordinates 75706..76122 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q84A07
Locus tag LRS40_RS00420 Protein ID WP_001261276.1
Coordinates 76119..76349 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LRS40_RS00395 (LRS40_00395) 71788..72573 + 786 WP_022650060.1 helix-turn-helix transcriptional regulator -
LRS40_RS00400 (LRS40_00400) 73167..73268 + 102 Protein_79 Tn3 family transposase -
LRS40_RS00405 (LRS40_00405) 73288..74364 - 1077 WP_000227969.1 IS110 family transposase -
LRS40_RS00410 (LRS40_00410) 74646..75502 - 857 Protein_81 IS3-like element ISEc15 family transposase -
LRS40_RS00415 (LRS40_00415) 75706..76122 - 417 WP_000754566.1 type II toxin-antitoxin system VapC family toxin Toxin
LRS40_RS00420 (LRS40_00420) 76119..76349 - 231 WP_001261276.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
LRS40_RS00425 (LRS40_00425) 76898..78325 - 1428 WP_231311747.1 phosphotransferase -
LRS40_RS00430 (LRS40_00430) 78306..79232 - 927 WP_231311748.1 hypothetical protein -
LRS40_RS00435 (LRS40_00435) 79229..81067 - 1839 WP_231311749.1 AAA family ATPase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid ant(3'')-Ia / blaTEM-1B / qnrS1 / floR / blaLAP-2 / tet(A) / dfrA1 / qacE / sul1 / tet(X4) / lnu(G) - 1..253593 253593


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15006.52 Da        Isoelectric Point: 9.2957

>T227012 WP_000754566.1 NZ_CP088263:c76122-75706 [Leclercia sp. G3L]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAVLPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNVREFARVPGLVLEDWVK

Download         Length: 417 bp

>T227012 NZ_CP122679:2196839-2196942 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTTTCTTTTT

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8721.74 Da        Isoelectric Point: 4.3533

>AT227012 WP_001261276.1 NZ_CP088263:c76349-76119 [Leclercia sp. G3L]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT227012 NZ_CP122679:c2196980-2196709 [Escherichia coli]
AAAGTCAGCGAAGGGAATGCTTCTGGCTTTTAACAGATAAAAAGAAACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGCGGTGGCCATCAGCTTAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A387K1G3

Antitoxin

Source ID Structure
AlphaFold DB A0A387K023

References