Detailed information of TA system TA227000

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 1964409..1965992
• Replicon: chromosome
• Accession: NZ_CP088241
• Organism: Stenotrophomonas maltophilia strain 2013-SM15

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: LQ329_RS09170
• Protein ID: WP_114618723.1
• Coordinates: 1964670..1965992 (+)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: LQ329_RS09165
• Protein ID: WP_005407534.1
• Coordinates: 1964409..1964666 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LQ329_RS09155 (LQ329_09155)	1959754..1960389	-	636	WP_005407536.1	hypothetical protein	-
LQ329_RS09160 (LQ329_09160)	1960561..1964247	+	3687	WP_114618722.1	indolepyruvate ferredoxin oxidoreductase family protein	-
LQ329_RS09165 (LQ329_09165)	1964409..1964666	+	258	WP_005407534.1	helix-turn-helix transcriptional regulator	Antitoxin
LQ329_RS09170 (LQ329_09170)	1964670..1965992	+	1323	WP_114618723.1	type II toxin-antitoxin system HipA family toxin	Toxin
LQ329_RS09175 (LQ329_09175)	1965993..1966607	-	615	WP_111174323.1	DUF938 domain-containing protein	-
LQ329_RS09180 (LQ329_09180)	1967294..1968925	+	1632	WP_162792552.1	TldD/PmbA family protein	-
LQ329_RS09185 (LQ329_09185)	1968956..1970590	+	1635	WP_005407530.1	TldD/PmbA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (4-105)

Antitoxin

• Cro/C1-type helix-turn-helix domain (19-70)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49031.96 Da
• Isoelectric Point: 7.0868

>T227000 WP_114618723.1 NZ_CP088241:1964670-1965992 [Stenotrophomonas maltophilia]
MGTLQVWMNGVEVALWDDVRNGSSRLSYVSQWISSPQSRPLSLSLPLLPDGESHRGSVVNDYFDNLLPDNVAIRNRIRDR
FATRGSDTFDLLEAIGRDCVGAVQLLPPARPPEDIQQITCEVLDEAGVATILRQVTTGNLPGAPSRDDAFRISIAGAQEK
TGLLLHAGKWCIPTGNTPSTHIFKLPLGIVGNMRADMHQSVENEWLCMHLLSAFDIPVAETQMGRFNDQRALIVERFDRR
LSADRRWWLRLPQEDMCQVFGLPAARKYESDGGPGIVPIMDLLRQSEHPEDRAIFFKTQILFWMLAATDGHAKNFSVHLE
AGGRFRLTPVYDVLSVYPILGRGPNQLDPRNAQLAMAVHGKNRHRKLHLVRRRHWNETARACGVADGAEPWIKTLIAAVE
PAIQQVTRQLPDDFPSNVSEPIFNGLRAAAARLSSMAPEA

Nucleotide

• Length: 1323 bp

>T227000 NZ_CP122676:c4003939-4003541 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTATTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAGCCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGAAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9584.97 Da
• Isoelectric Point: 11.8468

>AT227000 WP_005407534.1 NZ_CP088241:1964409-1964666 [Stenotrophomonas maltophilia]
MDRPLHTVRTAQQLAPLMRAFRRQAGLSQAQLAERLGISRQAVGALERDPASASFERLMRIWAVLGLEVSLQQRSPQENT
SSLEW

Nucleotide

• Length: 258 bp

>AT227000 NZ_CP122676:c4004235-4003942 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAATCCAGCAAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CAGAACAAGAGGAGAAAAAACTGGTGAAGGCGCGCTTCCGTCCGAGTGCCGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA