Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3132308..3132962 Replicon chromosome
Accession NC_010473
Organism Escherichia coli str. K-12 substr. DH10B

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag ECDH10B_RS15585 Protein ID WP_000244777.1
Coordinates 3132308..3132715 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag ECDH10B_RS15590 Protein ID WP_000354046.1
Coordinates 3132696..3132962 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECDH10B_RS15565 (ECDH10B_3066) 3128265..3129998 - 1734 WP_000813200.1 single-stranded-DNA-specific exonuclease RecJ -
ECDH10B_RS15570 (ECDH10B_3067) 3130004..3130714 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
ECDH10B_RS15575 (ECDH10B_3068) 3130739..3131635 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
ECDH10B_RS15580 (ECDH10B_3069) 3131747..3132268 + 522 WP_001055874.1 flavodoxin FldB -
ECDH10B_RS15585 (ECDH10B_3070) 3132308..3132715 - 408 WP_000244777.1 toxin CptA Toxin
ECDH10B_RS15590 (ECDH10B_3071) 3132696..3132962 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
ECDH10B_RS15595 (ECDH10B_3072) 3133205..3134185 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
ECDH10B_RS15600 (ECDH10B_3073) 3134381..3135040 - 660 WP_000250274.1 hemolysin III family protein -
ECDH10B_RS15605 (ECDH10B_3074) 3135204..3135515 - 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
ECDH10B_RS15610 (ECDH10B_3075) 3135560..3136993 + 1434 WP_001336277.1 6-phospho-beta-glucosidase BglA -
ECDH10B_RS15615 (ECDH10B_3076) 3137050..3137793 - 744 WP_000951964.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T22700 WP_000244777.1 NC_010473:c3132715-3132308 [Escherichia coli str. K-12 substr. DH10B]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T22700 NC_010473:c3132715-3132308 [Escherichia coli str. K-12 substr. DH10B]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT22700 WP_000354046.1 NC_010473:c3132962-3132696 [Escherichia coli str. K-12 substr. DH10B]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT22700 NC_010473:c3132962-3132696 [Escherichia coli str. K-12 substr. DH10B]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References