Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) ykfI-yfjZ/CbtA-CbeA
Location 4034808..4035487 Replicon chromosome
Accession NZ_CP088229
Organism Enterobacter kobei strain Ek72

Toxin (Protein)


Gene name ykfI Uniprot ID A0A411GL63
Locus tag LK774_RS19295 Protein ID WP_016243829.1
Coordinates 4034808..4035149 (-) Length 114 a.a.

Antitoxin (Protein)


Gene name yfjZ Uniprot ID A0A8B2XJW9
Locus tag LK774_RS19300 Protein ID WP_016243830.1
Coordinates 4035170..4035487 (-) Length 106 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LK774_RS19275 (LK774_19310) 4030082..4031827 + 1746 WP_014885284.1 DNA primase -
LK774_RS19280 (LK774_19315) 4031993..4033837 + 1845 WP_014885285.1 RNA polymerase sigma factor RpoD -
LK774_RS19285 (LK774_19320) 4033939..4034445 - 507 WP_014885286.1 G/U mismatch-specific DNA glycosylase -
LK774_RS19295 (LK774_19330) 4034808..4035149 - 342 WP_016243829.1 TA system toxin CbtA family protein Toxin
LK774_RS19300 (LK774_19335) 4035170..4035487 - 318 WP_016243830.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
LK774_RS19305 (LK774_19340) 4035508..4035729 - 222 WP_007777714.1 DUF987 domain-containing protein -
LK774_RS19310 (LK774_19345) 4035738..4036214 - 477 WP_016243831.1 RadC family protein -
LK774_RS19315 (LK774_19350) 4036230..4036688 - 459 WP_016243832.1 antirestriction protein -
LK774_RS19320 (LK774_19355) 4036790..4037029 - 240 WP_016243833.1 DUF905 domain-containing protein -
LK774_RS19325 (LK774_19360) 4037106..4037573 - 468 WP_016239439.1 protein YkfB -
LK774_RS19330 (LK774_19365) 4037597..4038040 - 444 WP_016243834.1 hypothetical protein -
LK774_RS19335 (LK774_19370) 4038040..4038276 - 237 WP_001115854.1 protein YpjK -
LK774_RS19340 (LK774_19375) 4038313..4039014 - 702 WP_047749107.1 WYL domain-containing protein -
LK774_RS19345 (LK774_19380) 4039231..4040052 - 822 WP_008324532.1 DUF932 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(5-104)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13020.04 Da        Isoelectric Point: 9.9961

>T226997 WP_016243829.1 NZ_CP088229:c4035149-4034808 [Enterobacter kobei]
MKTLPATTQRAVKPCLSPVAVWQMLLTRLLEQHYGLTLNDTPFSEERVIQEHIDAGISLADAVNFLVEKYELVRIDRKGF
NWQEQSPYLRAVDILRARQATGLLRQSRKNSVR

Download         Length: 342 bp

>T226997 NZ_CP122676:3348046-3348432 [Escherichia coli]
ATGGGTGTGTATAAGGCAAGACGATTCAGCCAAAGCACGAAAAAACTTGGTATTCACGATAAAGTTCTCATGGCTGCCGC
TGAAGAAGTGATGCAGGGCATATGGGAGGCTGATTTAGGTAGCGGTGTCATCAAAAAACGTTTACCCCTCCAACAAGGAA
AGAGTGGTGGAGCCAGAACAATTATCTTTTTTAAATCAGCAAATCATGTGTTTTTTTATGATGGTTGGTCCAAGAGTGGT
CTAAGCAGTAAAGGAACCAAAGAAATCGAAGATGATGAACTGGCGGCCTACAAAAAAATGGCAAATGCATTTCTGGCCTT
TAGCACTAAGCAAATAGAGGATTTGATCGAAACTGGCTTTTTGATTGAGGTGAAAAATGAGCGATAA

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 12041.72 Da        Isoelectric Point: 7.7518

>AT226997 WP_016243830.1 NZ_CP088229:c4035487-4035170 [Enterobacter kobei]
MTHITWGLQRNITPRLGARLVQEGNRMHYLADRASITGKFSDTECRKLDETFPHFIRQMESMLTTGEMNPRNAHCVTLFH
NGFTCEADTLGSCGYVYIAIYPTQR

Download         Length: 318 bp

>AT226997 NZ_CP122676:3348422-3348751 [Escherichia coli]
ATGAGCGATAAATTACAAATGATGCTGGAAGATGCAAAGGAACTGAATGCACTGGGCCTGATGTCTGATAGTGAAGTTGA
TGCCATTGCCGTCATGGTCAAAGCTCGTGAAGTCAGCGCCCGTATAGCCAAAGTCAAATCAATGACGGGTGAAGAGATCA
AAGCCATCCGCACCCGATACGGCATGTCACAATCCGTGCTGGCACATACAATGGGAATGTCAAAAGAAAGTGTCTCGAAA
TGGGAACGTAATGAAATAAAACCGAGCGGCCCGGCATTACGTATTTTGAATACATTGGCAATAAAAGGGCCGGAAGTTTT
TGCAAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A411GL63

Antitoxin

Source ID Structure
AlphaFold DB A0A8B2XJW9

References