Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3819413..3820070 Replicon chromosome
Accession NZ_CP088229
Organism Enterobacter kobei strain Ek72

Toxin (Protein)


Gene name cptA Uniprot ID A0A2W0G6Z0
Locus tag LK774_RS18275 Protein ID WP_014885136.1
Coordinates 3819413..3819823 (-) Length 137 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID V3PWU7
Locus tag LK774_RS18280 Protein ID WP_010435322.1
Coordinates 3819804..3820070 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LK774_RS18255 (LK774_18285) 3815407..3817140 - 1734 WP_023331239.1 single-stranded-DNA-specific exonuclease RecJ -
LK774_RS18260 (LK774_18290) 3817146..3817859 - 714 WP_014885133.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
LK774_RS18265 (LK774_18295) 3817888..3818784 - 897 WP_014885134.1 site-specific tyrosine recombinase XerD -
LK774_RS18270 (LK774_18300) 3818886..3819407 + 522 WP_014885135.1 flavodoxin FldB -
LK774_RS18275 (LK774_18305) 3819413..3819823 - 411 WP_014885136.1 protein YgfX Toxin
LK774_RS18280 (LK774_18310) 3819804..3820070 - 267 WP_010435322.1 FAD assembly factor SdhE Antitoxin
LK774_RS18285 (LK774_18315) 3820365..3821345 + 981 WP_023331241.1 tRNA-modifying protein YgfZ -
LK774_RS18290 (LK774_18320) 3821430..3822089 - 660 WP_023331242.1 hemolysin III family protein -
LK774_RS18295 (LK774_18325) 3822355..3823086 + 732 WP_014885139.1 MurR/RpiR family transcriptional regulator -
LK774_RS18300 (LK774_18330) 3823203..3824636 + 1434 WP_249979658.1 6-phospho-beta-glucosidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-132)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 137 a.a.        Molecular weight: 16135.04 Da        Isoelectric Point: 10.7510

>T226996 WP_014885136.1 NZ_CP088229:c3819823-3819413 [Enterobacter kobei]
VVLWQSDLRVSWRSQWMSLMLHGLVAAIVLLMPWPLSYTPLWLLLLSFVVFDSVRSQRRINARQGEIKLLMDSRLRWQGK
EWDILGMPWMLNSGMMLRLRNVDGGRCQHLWLAADSMDASEWRDLRRMLLQQPTQE

Download         Length: 411 bp

>T226996 NZ_CP122676:c3076489-3076112 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTACGGCCTCACACTGAATAACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCACTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCACTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCCCGCCGGGCGACAGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAACATCCGGGGGCGAAACAATGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10555.02 Da        Isoelectric Point: 5.1340

>AT226996 WP_010435322.1 NZ_CP088229:c3820070-3819804 [Enterobacter kobei]
MDINNKARIHWACRRGMRELDISIMPFFEYEYDSLSDDDKRLFVRLLESDDPDLFNWLMNHGKPADTELQRMVQLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT226996 NZ_CP122676:c3076946-3076578 [Escherichia coli]
GTGTCAGACACACTCTCCGGGACAACGCATCCCGATGACAATCATGACCGTCCCTGGTGGGGACTACCCTGCACCGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGCAATCAGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCGTACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAGCTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCCAGAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCAAAATGAAAAATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2W0G6Z0

Antitoxin

Source ID Structure
AlphaFold DB V3PWU7

References