Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1059606..1060226 Replicon chromosome
Accession NZ_CP088229
Organism Enterobacter kobei strain Ek72

Toxin (Protein)


Gene name Hha Uniprot ID A0A2W0G488
Locus tag LK774_RS05000 Protein ID WP_014882802.1
Coordinates 1059606..1059824 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID V3PBI9
Locus tag LK774_RS05005 Protein ID WP_008499288.1
Coordinates 1059852..1060226 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LK774_RS04970 (LK774_04980) 1055615..1055875 + 261 WP_010428154.1 type B 50S ribosomal protein L31 -
LK774_RS04975 (LK774_04985) 1055878..1056018 + 141 WP_003859006.1 type B 50S ribosomal protein L36 -
LK774_RS04980 (LK774_04990) 1056015..1056725 - 711 WP_014882798.1 GNAT family protein -
LK774_RS04985 (LK774_04995) 1056827..1058287 + 1461 Protein_966 PLP-dependent aminotransferase family protein -
LK774_RS04990 (LK774_05000) 1058259..1058726 - 468 WP_014882800.1 YlaC family protein -
LK774_RS04995 (LK774_05005) 1058845..1059396 - 552 WP_014882801.1 maltose O-acetyltransferase -
LK774_RS05000 (LK774_05010) 1059606..1059824 - 219 WP_014882802.1 HHA domain-containing protein Toxin
LK774_RS05005 (LK774_05015) 1059852..1060226 - 375 WP_008499288.1 Hha toxicity modulator TomB Antitoxin
LK774_RS05010 (LK774_05020) 1060738..1063884 - 3147 WP_112020553.1 multidrug efflux RND transporter permease subunit AcrB -
LK774_RS05015 (LK774_05025) 1063907..1065100 - 1194 WP_014882804.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-114)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8612.00 Da        Isoelectric Point: 8.9008

>T226985 WP_014882802.1 NZ_CP088229:c1059824-1059606 [Enterobacter kobei]
MSEKPLTKVDYLMRLRRCQSIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFVR

Download         Length: 219 bp

>T226985 NZ_CP122676:c829116-828739 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCACGCCCGTCTCCGGTTGAAATCTGGCAGACACTGCTCAC
CCGACTGCTGGATCAGCACTACGGCCTTACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGCGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTACGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGTTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCACGCCGGGCAACCGGCCTGATGACACGCGA
CAACTACAGAACGGTAAATAACATTACCCTGGGTAAACATCCGGGGGCGAAACAATGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14481.28 Da        Isoelectric Point: 4.8886

>AT226985 WP_008499288.1 NZ_CP088229:c1060226-1059852 [Enterobacter kobei]
MDEYSPKRHDIAQLKFLCESLYHDCLANLDESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINAQDLQKWRKSGNRLFRCFVNVSRANPVSLSC

Download         Length: 375 bp

>AT226985 NZ_CP122676:c829574-829206 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACGCTTCCCGACGACAATAACGACCGCACCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTTGGTGCCCGTCTGGTACAGGAAGGCAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGGCGGT
TCAGCGACGCGGATTCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGTTG
TGGCTACGTTTATATGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2W0G488

Antitoxin

Source ID Structure
AlphaFold DB V3PBI9

References