22698

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yfjZ-ypjF/CbtA-CbeA
Location 2866902..2867569 Replicon chromosome
Accession NC_010473
Organism Escherichia coli str. K-12 substr. DH10B

Toxin (Protein)


Gene name ypjF Uniprot ID Q46953
Locus tag ECDH10B_RS14305 Protein ID WP_001094400.1
Coordinates 2867240..2867569 (+) Length 110 a.a.

Antitoxin (Protein)


Gene name yfjZ Uniprot ID P52141
Locus tag ECDH10B_RS14300 Protein ID WP_000072690.1
Coordinates 2866902..2867219 (+) Length 106 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECDH10B_RS14280 2861954..2862963 - 1010 Protein_2730 arsenic transporter -
ECDH10B_RS14285 (ECDH10B_2809) 2863105..2864808 + 1704 WP_000896263.1 hypothetical protein -
ECDH10B_RS24540 2865380..2865603 + 224 Protein_2732 DUF905 family protein -
ECDH10B_RS14290 (ECDH10B_2810) 2865706..2866164 + 459 WP_000211841.1 antirestriction protein -
ECDH10B_RS14295 (ECDH10B_2811) 2866173..2866655 + 483 WP_001407480.1 RadC family protein -
ECDH10B_RS24545 (ECDH10B_2812) 2866664..2866864 + 201 WP_001395452.1 DUF987 domain-containing protein -
ECDH10B_RS14300 (ECDH10B_2813) 2866902..2867219 + 318 WP_000072690.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
ECDH10B_RS14305 (ECDH10B_2814) 2867240..2867569 + 330 WP_001094400.1 type IV toxin-antitoxin system toxin YpjF Toxin
ECDH10B_RS23395 2867933..2872513 - 4581 WP_010723187.1 adhesin-like autotransporter YpjA/EhaD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-108)

Antitoxin

Antitoxin CbeA

(5-104)


Sequences

Toxin        


Download         Length: 110 a.a.        Molecular weight: 12308.21 Da        Isoelectric Point: 7.2761

>T22698 WP_001094400.1 NC_010473:2867240-2867569 [Escherichia coli str. K-12 substr. DH10B]
MNTLPATISQAAKPCLSPVAVWQMLLTRLLEQHYGLTLNDTPFSDETVIKEHIDAGITLADAVNFLVEKYELVRIDHRGF
SWQQQSPYISVVDILRARRSTGLLKTNVK

Download         Length: 330 bp

>T22698 NC_010473:2867240-2867569 [Escherichia coli str. K-12 substr. DH10B]
ATGAACACTCTACCTGCTACAATTTCGCAGGCGGCGAAGCCCTGCCTGTCGCCAGTGGCTGTCTGGCAAATGCTACTGAC
ACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCG
ATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAACTGGTACGTATCGATCACAGAGGATTT
TCGTGGCAACAACAGTCTCCATATATTTCCGTAGTAGATATTCTGCGAGCAAGGCGCTCTACCGGCTTGCTAAAAACTAA
TGTGAAATAA

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 11737.27 Da        Isoelectric Point: 6.4630

>AT22698 WP_000072690.1 NC_010473:2866902-2867219 [Escherichia coli str. K-12 substr. DH10B]
MSNTTWGLQRDITPRLGARLVQEGNQLHYLADRASITGKFSDAECPKLDVVFPHFISQIESMLTTGELNPRHAQCVTLYH
NGFTCEADTLGSCGYVYIAVYPTQR

Download         Length: 318 bp

>AT22698 NC_010473:2866902-2867219 [Escherichia coli str. K-12 substr. DH10B]
ATGAGCAACACCACATGGGGCCTGCAGCGAGATATCACGCCGCGCCTGGGAGCACGTCTGGTGCAGGAGGGCAACCAGCT
GCACTATCTGGCTGACCGGGCCAGTATCACCGGTAAGTTTAGTGACGCCGAATGTCCTAAGCTGGATGTGGTATTTCCAC
ATTTTATCAGCCAGATAGAGTCGATGCTGACCACTGGTGAACTGAATCCCCGCCATGCCCAATGCGTCACCCTGTACCAC
AACGGTTTTACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGTATACATCGCTGTTTACCCCACTCAACGCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A373F4I3

Antitoxin

Source ID Structure
PDB 2EA9
PDB 2JN7
AlphaFold DB P52141

References