Detailed information of TA system TA226952

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): omega-epsilon-zeta/zeta-epsilon
• Location: 27859..28996
• Replicon: plasmid pS39-4-b
• Accession: NZ_CP088202
• Organism: Enterococcus faecalis strain S39-4

Toxin (Protein)

• Gene name: zeta
• Uniprot ID: -
• Locus tag: LQ044_RS14660
• Protein ID: WP_100185210.1
• Coordinates: 27859..28722 (-)
• Length: 288 a.a.

Antitoxin (Protein)

• Gene name: epsilon
• Uniprot ID: R2XCR7
• Locus tag: LQ044_RS14665
• Protein ID: WP_000301765.1
• Coordinates: 28724..28996 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LQ044_RS14625 (LQ044_14625)	23011..23343	-	333	WP_000713503.1	CagC family type IV secretion system protein	-
LQ044_RS14630 (LQ044_14630)	23367..25352	-	1986	WP_000974115.1	MobQ family relaxase	-
LQ044_RS14635 (LQ044_14635)	25644..25943	+	300	WP_000431202.1	hypothetical protein	-
LQ044_RS14640 (LQ044_14640)	25946..26203	+	258	WP_000002668.1	hypothetical protein	-
LQ044_RS14645 (LQ044_14645)	26226..26531	-	306	WP_000351790.1	hypothetical protein	-
LQ044_RS14650 (LQ044_14650)	26565..27062	-	498	WP_249469435.1	molecular chaperone DnaJ	-
LQ044_RS14655 (LQ044_14655)	27101..27418	-	318	WP_002338433.1	hypothetical protein	-
LQ044_RS14660 (LQ044_14660)	27859..28722	-	864	WP_100185210.1	zeta toxin family protein	Toxin
LQ044_RS14665 (LQ044_14665)	28724..28996	-	273	WP_000301765.1	antitoxin	Antitoxin
LQ044_RS14670 (LQ044_14670)	29013..29228	-	216	WP_002295735.1	peptide-binding protein	-
LQ044_RS14675 (LQ044_14675)	29327..30223	-	897	WP_002304405.1	ParA family protein	-
LQ044_RS14680 (LQ044_14680)	30326..32470	-	2145	WP_225001404.1	type IA DNA topoisomerase	-
LQ044_RS14685 (LQ044_14685)	32470..33087	-	618	WP_002295591.1	recombinase family protein	-
LQ044_RS14690 (LQ044_14690)	33101..33271	-	171	WP_000713595.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	Cfr(D) / poxtA / fexA	-	1..33618	33618

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (27-214)

Antitoxin

• Bacterial epsilon antitoxin (2-90)

Sequences

Toxin

Protein

• Length: 288 a.a.
• Molecular weight: 32416.95 Da
• Isoelectric Point: 6.6610

>T226952 WP_100185210.1 NZ_CP088202:c28722-27859 [Enterococcus faecalis]
MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGSGKTSLRSAILEETQGNVIVIDNDTFKQQHPNFDELA
KLYEKDVVKHVTPYSNRMTEAIISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETPSISPKETLERELNRKVSGKEIQ
PTLERIEQKMLQNQHQETPEFKAIQQKMESLQPPTPPIPKTPKLPGL

Nucleotide

• Length: 864 bp

>T226952 NZ_CP122660:3532011-3532229 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10721.25 Da
• Isoelectric Point: 6.4676

>AT226952 WP_000301765.1 NZ_CP088202:c28996-28724 [Enterococcus faecalis]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEVNLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYS
KQVLNKEKVA

Nucleotide

• Length: 273 bp

>AT226952 NZ_CP122660:3531611-3531985 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Antitoxin

• AlphaFold DB: A0A829F0A3