Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 2766519..2767090 Replicon chromosome
Accession NZ_CP088200
Organism Enterococcus faecalis strain S39-4

Toxin (Protein)


Gene name mazF Uniprot ID R3GRA7
Locus tag LQ044_RS13640 Protein ID WP_002360937.1
Coordinates 2766519..2766860 (-) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID R3JGB1
Locus tag LQ044_RS13645 Protein ID WP_002354773.1
Coordinates 2766860..2767090 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LQ044_RS13635 (2762534) 2762534..2766148 - 3615 WP_002389470.1 DNA-directed RNA polymerase subunit beta -
LQ044_RS13640 (2766519) 2766519..2766860 - 342 WP_002360937.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
LQ044_RS13645 (2766860) 2766860..2767090 - 231 WP_002354773.1 hypothetical protein Antitoxin
LQ044_RS13650 (2767497) 2767497..2767712 - 216 WP_002354768.1 zinc ribbon domain-containing protein -
LQ044_RS13655 (2767851) 2767851..2768843 + 993 WP_002354765.1 biotin--[acetyl-CoA-carboxylase] ligase -
LQ044_RS13660 (2769107) 2769107..2769736 - 630 WP_002354763.1 lytic polysaccharide monooxygenase -
LQ044_RS13665 (2770429) 2770429..2772045 + 1617 WP_002381036.1 phosphatase PAP2/LCP family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-112)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13165.41 Da        Isoelectric Point: 9.3988

>T226933 WP_002360937.1 NZ_CP088200:c2766860-2766519 [Enterococcus faecalis]
MNVEKKYIPKKGDIVWIDFDPAAGKEIQKRRPGLVVSRYEFNRKTMFAVICPITSTIKNIPTRYTLPDDIETHGQVVISQ
LKSLDFTERKLSQIEHLPLKDMAKIDQIIEYIF

Download         Length: 342 bp

>T226933 NZ_CP122655:c4494315-4494142 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8677.67 Da        Isoelectric Point: 4.3313

>AT226933 WP_002354773.1 NZ_CP088200:c2767090-2766860 [Enterococcus faecalis]
MLTTKSRRQGSSVVLTLPTDNGKKPKVDQEYIVMYSDDGTITLVPKIQDPFSEGPEGEYYEKDEWHDLAPEGRELF

Download         Length: 231 bp

>AT226933 NZ_CP122655:c4494639-4494343 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGATT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M2A7G9

Antitoxin

Source ID Structure
AlphaFold DB A0A0M2A812

References