Detailed information of TA system TA226926

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ImmA-IrrE/HTH_26(antitoxin)
• Location: 2427457..2428152
• Replicon: chromosome
• Accession: NZ_CP088200
• Organism: Enterococcus faecalis strain S39-4

Toxin (Protein)

• Gene name: IrrE
• Uniprot ID: A0A059N529
• Locus tag: LQ044_RS12020
• Protein ID: WP_002373917.1
• Coordinates: 2427808..2428152 (+)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: ImmA
• Uniprot ID: -
• Locus tag: LQ044_RS12015
• Protein ID: WP_002373919.1
• Coordinates: 2427457..2427789 (+)
• Length: 111 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LQ044_RS11965 (2422978)	2422978..2423793	-	816	WP_161973016.1	PD-(D/E)XK nuclease-like domain-containing protein	-
LQ044_RS11970 (2423756)	2423756..2424766	-	1011	WP_104885362.1	RecT family recombinase	-
LQ044_RS11975 (2424867)	2424867..2425091	-	225	WP_002370012.1	hypothetical protein	-
LQ044_RS11980 (2425088)	2425088..2425387	-	300	WP_010730100.1	hypothetical protein	-
LQ044_RS11985 (2425474)	2425474..2425890	-	417	WP_002417286.1	hypothetical protein	-
LQ044_RS11990 (2425891)	2425891..2426061	-	171	WP_010706857.1	hypothetical protein	-
LQ044_RS11995 (2426096)	2426096..2426365	-	270	WP_002365131.1	hypothetical protein	-
LQ044_RS12000 (2426383)	2426383..2426562	-	180	WP_229202013.1	DUF2829 domain-containing protein	-
LQ044_RS12005 (2426625)	2426625..2426963	-	339	WP_010706859.1	hypothetical protein	-
LQ044_RS12010 (2426976)	2426976..2427158	-	183	WP_002358110.1	hypothetical protein	-
LQ044_RS12015 (2427457)	2427457..2427789	+	333	WP_002373919.1	helix-turn-helix transcriptional regulator	Antitoxin
LQ044_RS12020 (2427808)	2427808..2428152	+	345	WP_002373917.1	ImmA/IrrE family metallo-endopeptidase	Toxin
LQ044_RS12025 (2428181)	2428181..2428579	+	399	WP_010706860.1	hypothetical protein	-
LQ044_RS12030 (2428597)	2428597..2429328	+	732	WP_010706861.1	potassium channel family protein	-
LQ044_RS12035 (2429483)	2429483..2430664	+	1182	WP_002365138.1	site-specific integrase	-
LQ044_RS12040 (2430767)	2430767..2430916	-	150	WP_002356321.1	50S ribosomal protein L33	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2386287..2430664	44377

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• IrrE N-terminal-like domain (21-86)

Antitoxin

• Cro/C1-type helix-turn-helix domain (5-59)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 13594.61 Da
• Isoelectric Point: 5.6177

>T226926 WP_002373917.1 NZ_CP088200:2427808-2428152 [Enterococcus faecalis]
MKSIKELVEEYNVELVFTTLNKRACFDPTYGIIFVNQNLTPSEQEEAIYHELKHVKDHVDIMALYKIPVFRSKMEAEAEQ
YMFRSLIEKYEGQYNYSNVIAHYNLKMGQEIYLK

Nucleotide

• Length: 345 bp

>T226926 NZ_CP122655:c2231629-2231342 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin

Protein

• Length: 111 a.a.
• Molecular weight: 12734.28 Da
• Isoelectric Point: 5.2423

>AT226926 WP_002373919.1 NZ_CP088200:2427457-2427789 [Enterococcus faecalis]
MNTYEIIKELTKRKKMSIRQLEITLGYSNGYFSKWKKVSPNSEGLQKVADYFNVSVDYLLGRTDNTKATDEKNSDDLDDV
LDNVMSFDGEPLDDHDREVIRAYLKGRFGK

Nucleotide

• Length: 333 bp

>AT226926 NZ_CP122655:c2231868-2231629 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Structures

Toxin

• AlphaFold DB: A0A059N529