Detailed information of TA system TA226925

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ImmA-IrrE/Peptidase_M78-HTH_26
• Location: 1827154..1827852
• Replicon: chromosome
• Accession: NZ_CP088200
• Organism: Enterococcus faecalis strain S39-4

Toxin (Protein)

• Gene name: IrrE
• Uniprot ID: A0A828QMF1
• Locus tag: LQ044_RS09035
• Protein ID: WP_002388207.1
• Coordinates: 1827508..1827852 (+)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: ImmA
• Uniprot ID: -
• Locus tag: LQ044_RS09030
• Protein ID: WP_002388206.1
• Coordinates: 1827154..1827489 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LQ044_RS08980 (1822520)	1822520..1823461	-	942	WP_002364219.1	YqaJ viral recombinase family protein	-
LQ044_RS08985 (1823559)	1823559..1823786	-	228	WP_002364220.1	hypothetical protein	-
LQ044_RS08990 (1823786)	1823786..1824109	-	324	WP_002380444.1	hypothetical protein	-
LQ044_RS08995 (1824153)	1824153..1824338	-	186	WP_002364222.1	hypothetical protein	-
LQ044_RS09000 (1824329)	1824329..1824523	-	195	WP_002364223.1	hypothetical protein	-
LQ044_RS09005 (1824576)	1824576..1824758	+	183	WP_002364224.1	YegP family protein	-
LQ044_RS09010 (1824798)	1824798..1825523	-	726	WP_025187960.1	ORF6C domain-containing protein	-
LQ044_RS09015 (1825562)	1825562..1825873	-	312	WP_002381719.1	hypothetical protein	-
LQ044_RS09020 (1826470)	1826470..1826667	-	198	WP_010712611.1	hypothetical protein	-
LQ044_RS09025 (1826680)	1826680..1826862	-	183	WP_002388205.1	hypothetical protein	-
LQ044_RS09030 (1827154)	1827154..1827489	+	336	WP_002388206.1	helix-turn-helix transcriptional regulator	Antitoxin
LQ044_RS09035 (1827508)	1827508..1827852	+	345	WP_002388207.1	ImmA/IrrE family metallo-endopeptidase	Toxin
LQ044_RS09040 (1827910)	1827910..1828638	+	729	WP_002388208.1	potassium channel family protein	-
LQ044_RS09045 (1828738)	1828738..1829886	+	1149	WP_002388210.1	site-specific integrase	-
LQ044_RS09050 (1829914)	1829914..1830356	-	443	Protein_1758	competence type IV pilus minor pilin ComGD	-
LQ044_RS09055 (1830353)	1830353..1830628	-	276	WP_002356991.1	competence type IV pilus major pilin ComGC	-
LQ044_RS09060 (1830628)	1830628..1831674	-	1047	WP_002356990.1	competence type IV pilus assembly protein ComGB	-
LQ044_RS09065 (1831631)	1831631..1832599	-	969	WP_002364362.1	competence type IV pilus ATPase ComGA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1790063..1848735	58672

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• IrrE N-terminal-like domain (21-86)

Antitoxin

• Cro/C1-type helix-turn-helix domain (5-60)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 13625.58 Da
• Isoelectric Point: 4.9570

>T226925 WP_002388207.1 NZ_CP088200:1827508-1827852 [Enterococcus faecalis]
MKSIKELVEEYNVELVFTTLNKRACFDPTYGIIFVNQNLTPTEQEEAIYHELKHVEDHVDIMALYKIPVFRSKMESEAEQ
YMFRSLIEKYEGQYNYSNVIAHYNLKMGQEIYLK

Nucleotide

• Length: 345 bp

>T226925 NZ_CP122655:c2156358-2155036 [Escherichia coli]
ATGCCTAAACTTGTCACTTGGATGAACAACCAGCGGGTAGGCGAGTTAACGAAGTTAGCCAACGGCGCGCACACCTTTAA
GTATGCACCGGAGTGGTTAGCAAGCCGTTATGCCAGACCGTTGTCACTTTCGCTGCCATTGCAGAGGGGGAATATCACCT
CTGATGCCGTATTTAACTTCTTCGATAACCTGTTACCCGATAGCCCGATTGTACGTGACCGGATCGTTAAACGTTATCAT
GCCAAATCCAGACAACCGTTTGATTTATTGTCAGAAATAGGGCGAGACAGCGTTGGTGCCGTGACGTTAATACCCGAAGA
CGAAACCGTAACGCATCCGATAATGGCATGGGAAAAGCTTACTGAAGCCAGACTTGAAGAAGTATTAACGGCTTATAAAG
CAGATATCCCGCTAGGCATGATTAGAGAAGAAAATGACTTTCGCATCTCGGTTGCTGGCGCACAGGAGAAGACAGCACTG
CTCAGAATAGGCAATGACTGGTGCATTCCGAAAGGAATAACGCCGACGACGCACATCATTAAATTACCGATTGGCGAAAT
CAGGCAGCCCAATGCGACGCTCGATCTCAGCCAAAGCGTTGATAATGAGTATTACTGTCTGCTGCTGGCGAAAGAACTTG
GGTTGAATGTTCCGGACGCAGAAATCATTAAAGCGGGAAATGTGCGCGCGTTAGCGGTCGAACGTTTTGACAGGCGTTGG
AATGCTGAGCGAACGGTTTTACTTCGCTTGCCACAGGAGGATATGTGTCAGACATTCGGTTTACCTTCATCGGTGAAATA
TGAATCAGATGGAGGCCCAGGCATCGCGCGGATCATGGCTTTTTTGATGGGGTCCAGCGAGGCGCTGAAAGATCGCTATG
ATTTTATGAAATTCCAGGTCTTCCAGTGGTTGATTGGCGCAACGGACGGTCATGCAAAAAACTTCTCCGTATTTATTCAG
GCTGGCGGCAGTTATCGACTCACGCCATTTTACGACATCATTTCAGCATTTCCGGTCCTTGGCGGTACGGGAATACACAT
CAGCGATCTCAAACTGGCAATGGGGCTTAACGCATCCAAAGGCAAAAAAACGGCAATCGATAAAATTTATCCGCGACATT
TTTTGGCGACAGCAAAGGTGCTGAGATTCCCGGAAGTGCAGATGCATGAAATCCTGAGTGACTTTGCCAGAATGATTCCA
GCAGCACTGGATAACGTGAAGACTTCATTACCGACAGATTTTCCGGAGAACGTGGTGACGGCAGTTGAAAGCAATGTGTT
GAGGTTGCATGGACGGTTAAGCCGAGAATACGGTAGTAAGTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12842.51 Da
• Isoelectric Point: 5.2926

>AT226925 WP_002388206.1 NZ_CP088200:1827154-1827489 [Enterococcus faecalis]
MTVFDRIKLLAKERGKSLNRIEEELGLPKNVLYRMKTSDNPTKDRLETLANYFDVSVDYLLGRTDNTKATDEKKSDDLDD
VLDNVMSFDGKPLDDHDREVIRAYLKGRFGK

Nucleotide

• Length: 336 bp

>AT226925 NZ_CP122655:c2156624-2156358 [Escherichia coli]
ATGATGAGCTTTCAGAAGATCTATAGCCCAACGCAATTGGCGAATGCAATGAAACTGGTTCGCCAGCAAAATGGCTGGAC
GCAGAGCGAGCTGGCGAAAAAAATTGGTATTAAGCAGGCGACGATTTCCAATTTCGAAAACAACCCTGACAATACCACGC
TCACGACATTTTTTAAGATTTTACAGTCGCTTGAACTCTCAATGACGCTATGCGACGCGAAAAATGCCTCGCCAGAATCA
ACAGAACAGCAAAATCTGGAGTGGTAA

Structures

Toxin

• AlphaFold DB: A0A828QMF1