Detailed information of TA system TA226921

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): omega-epsilon-zeta/zeta-epsilon
• Location: 417225..417807
• Replicon: chromosome
• Accession: NZ_CP088200
• Organism: Enterococcus faecalis strain S39-4

Toxin (Protein)

• Gene name: zeta
• Uniprot ID: A0A0M2AE74
• Locus tag: LQ044_RS02140
• Protein ID: WP_002355414.1
• Coordinates: 417499..417807 (+)
• Length: 103 a.a.

Antitoxin (Protein)

• Gene name: epsilon
• Uniprot ID: Q9AL19
• Locus tag: LQ044_RS02135
• Protein ID: WP_002326825.1
• Coordinates: 417225..417497 (+)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LQ044_RS02105 (412506)	412506..413234	-	729	WP_002355404.1	GntR family transcriptional regulator	-
LQ044_RS02110 (413411)	413411..414337	+	927	WP_002355406.1	hypothetical protein	-
LQ044_RS02115 (414354)	414354..415637	+	1284	WP_002363033.1	PTS sugar transporter subunit IIC	-
LQ044_RS02120 (415829)	415829..415951	+	123	Protein_394	topoisomerase	-
LQ044_RS02125 (416026)	416026..416922	+	897	WP_002363034.1	ParA family protein	-
LQ044_RS02130 (416999)	416999..417208	+	210	WP_002363035.1	peptide-binding protein	-
LQ044_RS02135 (417225)	417225..417497	+	273	WP_002326825.1	antitoxin	Antitoxin
LQ044_RS02140 (417499)	417499..417807	+	309	WP_002355414.1	zeta toxin family protein	Toxin
LQ044_RS02145 (417887)	417887..418309	-	423	WP_080005963.1	tyrosine-type recombinase/integrase	-
LQ044_RS02150 (418360)	418360..418860	-	501	WP_002363039.1	HAD family hydrolase	-
LQ044_RS02155 (418865)	418865..419632	-	768	WP_002355416.1	DeoR/GlpR family DNA-binding transcription regulator	-
LQ044_RS02160 (420120)	420120..420545	+	426	WP_002355418.1	galactose-6-phosphate isomerase subunit LacA	-
LQ044_RS02165 (420562)	420562..421077	+	516	WP_002345825.1	galactose-6-phosphate isomerase subunit LacB	-
LQ044_RS02170 (421088)	421088..422020	+	933	WP_002363040.1	tagatose-6-phosphate kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	ClpL	bsh / esp / psaA	385040..529997	144957

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (27-99)

Antitoxin

• Bacterial epsilon antitoxin (2-90)

Sequences

Toxin

Protein

• Length: 103 a.a.
• Molecular weight: 11525.91 Da
• Isoelectric Point: 5.7251

>T226921 WP_002355414.1 NZ_CP088200:417499-417807 [Enterococcus faecalis]
MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGSGKTSLRSAISEETQGNVVIIDNDTFKQQHPNFDELV
KLYEKDVVKHATSYSNQLVKLN

Nucleotide

• Length: 309 bp

>T226921 NZ_CP122655:874841-875248 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10720.17 Da
• Isoelectric Point: 4.4865

>AT226921 WP_002326825.1 NZ_CP088200:417225-417497 [Enterococcus faecalis]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEVNLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYS
KQILTKEKVA

Nucleotide

• Length: 273 bp

>AT226921 NZ_CP122655:874594-874860 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: A0A0M2AE74

Antitoxin

• AlphaFold DB: A0A0M2AF93