Detailed information of TA system TA226897

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Xre-MNT/HTH_26(antitoxin)
• Location: 80597..81705
• Replicon: plasmid pS2-6b-a
• Accession: NZ_CP088192
• Organism: Enterococcus faecium strain S2-6b

Toxin (Protein)

• Gene name: MNTss
• Uniprot ID: R3JIN1
• Locus tag: LQ050_RS12605
• Protein ID: WP_000233000.1
• Coordinates: 80597..81466 (-)
• Length: 290 a.a.

Antitoxin (Protein)

• Gene name: Xress
• Uniprot ID: -
• Locus tag: LQ050_RS12610
• Protein ID: WP_000205227.1
• Coordinates: 81481..81705 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LQ050_RS12575 (LQ050_12575)	75759..76439	+	681	WP_001015311.1	IS6-like element IS1216 family transposase	-
LQ050_RS12580 (LQ050_12580)	76667..77239	-	573	WP_202074493.1	HTH domain-containing protein	-
LQ050_RS12585 (LQ050_12585)	77515..78309	-	795	WP_001096887.1	aminoglycoside O-phosphotransferase APH(3')-IIIa	-
LQ050_RS12590 (LQ050_12590)	78402..78944	-	543	WP_000627290.1	streptothricin N-acetyltransferase Sat4	-
LQ050_RS12595 (LQ050_12595)	78941..79849	-	909	WP_001255866.1	aminoglycoside nucleotidyltransferase ANT(6)-Ia	-
LQ050_RS12600 (LQ050_12600)	79882..80616	-	735	WP_000662263.1	class I SAM-dependent methyltransferase	-
LQ050_RS12605 (LQ050_12605)	80597..81466	-	870	WP_000233000.1	nucleotidyltransferase domain-containing protein	Toxin
LQ050_RS12610 (LQ050_12610)	81481..81705	-	225	WP_000205227.1	helix-turn-helix transcriptional regulator	Antitoxin
LQ050_RS12615 (LQ050_12615)	81848..82077	-	230	Protein_88	hypothetical protein	-
LQ050_RS12620 (LQ050_12620)	82143..83425	+	1283	Protein_89	ISL3 family transposase	-
LQ050_RS12625 (LQ050_12625)	83734..84537	-	804	WP_002294514.1	lincosamide nucleotidyltransferase Lnu(B)	-
LQ050_RS12630 (LQ050_12630)	84591..86075	-	1485	WP_002294513.1	ABC-F type ribosomal protection protein Lsa(E)	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polymerase, nucleotidyl transferase domain (12-111)

Antitoxin

• Cro/C1-type helix-turn-helix domain (4-57)

Sequences

Toxin

Protein

• Length: 290 a.a.
• Molecular weight: 32926.57 Da
• Isoelectric Point: 4.8781

>T226897 WP_000233000.1 NZ_CP088192:c81466-80597 [Enterococcus faecium]
MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIYYNSDSFDLTAINQIATELDDKNRNNLVVPPGAWGDW
INGGGWLFINGYHVDLILRDIKRVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
PTALKKSLMNFFIFEAEFSLMFVKANAGVEDKYYIAGHVFRIISCLNQVLFACNNAYCINEKKAIKLLETFEYKPEKYAE
RVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINNFLNEESSDERKQI

Nucleotide

• Length: 870 bp

>T226897 NZ_CP122652:959969-960376 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATCTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATCCACGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGCATTAATGCTCGCCAGGGGGAAATTCGCCTGTTGATGGACGGGCGTTTGCGCTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGACAGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATATTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8365.68 Da
• Isoelectric Point: 4.5510

>AT226897 WP_000205227.1 NZ_CP088192:c81705-81481 [Enterococcus faecium]
MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLDELKAIADVLGVKFVQAFILPDGEEIKISNE

Nucleotide

• Length: 225 bp

>AT226897 NZ_CP122652:959722-959988 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAGTGTGACGATCCCGATC
TGTTTAACTGGCTGATGAACCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: R3JIN1