Detailed information of TA system TA226894

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Xre-MNT/HTH_26(antitoxin)
• Location: 513359..514467
• Replicon: chromosome
• Accession: NZ_CP088183
• Organism: Enterococcus hirae strain S2-7

Toxin (Protein)

• Gene name: MNTss
• Uniprot ID: R3JIN1
• Locus tag: LQ051_RS02410
• Protein ID: WP_000233000.1
• Coordinates: 513598..514467 (+)
• Length: 290 a.a.

Antitoxin (Protein)

• Gene name: Xress
• Uniprot ID: -
• Locus tag: LQ051_RS02405
• Protein ID: WP_000205227.1
• Coordinates: 513359..513583 (+)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LQ051_RS02375 (LQ051_02375)	508820..509050	+	231	Protein_469	LytR family transcriptional regulator	-
LQ051_RS02380 (LQ051_02380)	509105..509785	-	681	WP_001015311.1	IS6-like element IS1216 family transposase	-
LQ051_RS02385 (LQ051_02385)	509876..510427	+	552	Protein_471	DNA topoisomerase	-
LQ051_RS02390 (LQ051_02390)	510738..511235	+	498	WP_002327635.1	DNA recombinase	-
LQ051_RS02395 (LQ051_02395)	511236..511652	+	417	WP_000323438.1	recombinase	-
LQ051_RS02400 (LQ051_02400)	511672..513216	+	1545	WP_002390960.1	recombinase family protein	-
LQ051_RS02405 (LQ051_02405)	513359..513583	+	225	WP_000205227.1	helix-turn-helix transcriptional regulator	Antitoxin
LQ051_RS02410 (LQ051_02410)	513598..514467	+	870	WP_000233000.1	nucleotidyltransferase domain-containing protein	Toxin
LQ051_RS02415 (LQ051_02415)	514448..515182	+	735	WP_000662263.1	class I SAM-dependent methyltransferase	-
LQ051_RS02420 (LQ051_02420)	515215..516078	+	864	WP_002294505.1	aminoglycoside nucleotidyltransferase ANT(6)-Ia	-
LQ051_RS02425 (LQ051_02425)	516122..516649	+	528	WP_002294507.1	phosphoribosyltransferase family protein	-
LQ051_RS02430 (LQ051_02430)	516877..517383	+	507	Protein_480	transposase	-
LQ051_RS02435 (LQ051_02435)	517439..518119	-	681	WP_002323195.1	IS6 family transposase	-
LQ051_RS02440 (LQ051_02440)	518732..519091	+	360	WP_249464131.1	helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polymerase, nucleotidyl transferase domain (12-111)

Antitoxin

• Cro/C1-type helix-turn-helix domain (4-57)

Sequences

Toxin

Protein

• Length: 290 a.a.
• Molecular weight: 32926.57 Da
• Isoelectric Point: 4.8781

>T226894 WP_000233000.1 NZ_CP088183:513598-514467 [Enterococcus hirae]
MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIYYNSDSFDLTAINQIATELDDKNRNNLVVPPGAWGDW
INGGGWLFINGYHVDLILRDIKRVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
PTALKKSLMNFFIFEAEFSLMFVKANAGVEDKYYIAGHVFRIISCLNQVLFACNNAYCINEKKAIKLLETFEYKPEKYAE
RVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINNFLNEESSDERKQI

Nucleotide

• Length: 870 bp

>T226894 NZ_CP122652:154236-154421 [Escherichia coli]
GTGAAAAGTGCAGACGTGATCGCCATCCTGAAGCAGCATGGCTGGGAACATATTCGAACCCGTGGCAGTCATCATCAGTT
CCGCCACCCTGTTCATCCGGGTCTGGTGACGGTTCCGCACCCTAAAAAAGATATTAAGCCCGGTACGCTGGCGCAAATTG
GGCGTCAGGCTGGTTTAACATTTTAA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8365.68 Da
• Isoelectric Point: 4.5510

>AT226894 WP_000205227.1 NZ_CP088183:513359-513583 [Enterococcus hirae]
MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLDELKAIADVLGVKFVQAFILPDGEEIKISNE

Nucleotide

• Length: 225 bp

>AT226894 NZ_CP122652:154438-154848 [Escherichia coli]
ATGTTTTATCCCGCCTATATCCACAGTGATCCTGACGGCAGTGCCAGCGGTTTTTTCCCTGATGTTCCAGGCTGTTATTT
TGCTGGTGATACGCTGGACTACGCTTTCCAGGATGCGCGAAGTGCACTGGTCGCGCACTTCGAAACCTTATGCGAGATGA
ATGAAGAGTTGCCGCTTCCAGGCTCTGTGGAAACACATCTGGTCCAGCGTGCGCAGGACTTTATTGGCGGGCAATGGTTG
CTGGTGGATATCAATATGAATCAGTTCGAAGGTCGGGCTGAACGTATCAATATTACGATGCCAAAAAGATTACTGAACAA
GATTGATACCTACGTGCGCAATAACCCAGACTATGCCAACAGAAGTGCGTTTCTGGCGGAAGCTGCCCGGCGTGTATTGC
CGGGCGTTTAA

Structures

Toxin

• AlphaFold DB: R3JIN1