Detailed information of TA system TA226886

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2081006..2081535
• Replicon: chromosome
• Accession: NZ_CP088158
• Organism: Staphylococcus aureus strain 697

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: LRP64_RS10180
• Protein ID: WP_000621175.1
• Coordinates: 2081006..2081368 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: LRP64_RS10185
• Protein ID: WP_000948331.1
• Coordinates: 2081365..2081535 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LRP64_RS10160 (2077985)	2077985..2078755	-	771	WP_001041107.1	RNA polymerase sigma factor SigB	-
LRP64_RS10165 (2078730)	2078730..2079209	-	480	WP_001190825.1	anti-sigma B factor RsbW	-
LRP64_RS10170 (2079211)	2079211..2079537	-	327	WP_001052491.1	anti-sigma factor antagonist	-
LRP64_RS10175 (2079656)	2079656..2080657	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
LRP64_RS10180 (2081006)	2081006..2081368	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
LRP64_RS10185 (2081365)	2081365..2081535	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
LRP64_RS10190 (2081620)	2081620..2082768	-	1149	WP_001280702.1	alanine racemase	-
LRP64_RS10195 (2082834)	2082834..2083193	-	360	WP_000581194.1	holo-ACP synthase	-
LRP64_RS10200 (2083197)	2083197..2083688	-	492	WP_001205915.1	PH domain-containing protein	-
LRP64_RS10205 (2083675)	2083675..2085258	-	1584	WP_001294642.1	PH domain-containing protein	-
LRP64_RS10210 (2085251)	2085251..2085730	-	480	WP_001287083.1	hypothetical protein	-
LRP64_RS10215 (2085939)	2085939..2086499	-	561	WP_001092405.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T226886 WP_000621175.1 NZ_CP088158:c2081368-2081006 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T226886 NZ_CP122648:c4020705-4019785 [Escherichia coli]
ATGGATCTGCGTGATAGTTATGCCCGGCAGGTTAAGCTGTTGATGGCTGCTCTGCCGCATGTGGCGAAGGAGTCCTGTTT
CGCGTTGAAAGGCGGCACGGCAATCAATTTGTTTGTGCAGGATTTTCCCCGTCTGTCGGTGGATATTGATCTGGCGTATA
AGAGCTTTGCGGATCGCGATACTGATCTGGCGGCTATCAACGACGCTTTGAAGAGGATTACTGCATCGCTGAACGGCAGA
CCGGGGATCACCGCGATTCGGCAGGAAAATAAAGCGGATGAAAAGCGCATCATTGTGAATACCGCTGATGCAAAGATTAA
GATCGAGGTTAGCCCGGTCTGGCGCGGGCTGTTGTTGCCTCCGGCAGAAATGCCTGTGTGCGAACGTGTGGAAATGGAGT
ATGGCTTCACCACCATGAGTGTCGTCTCGCTGGCGGATCTTTATGGCGGTAAAATCTGCGCAGCGCTTGATCGTCAGCAC
CCGCGCGATCTGTTTGATGTGCTGAATATGCTGGAGAAACCGGGACTGATGCGGGAGATCTTTGACGGTTTTCTTTGTTA
TCTGGCAGGCCATCCCCGCCCCATTGCCGAGTTGCTGGCCCCAAACTGGGATATAGAGCGGATCACGACGCTGTATGCTC
AGGAATTTTCGGGGATGACTCAGCAGGAAACGTCGCTGGAATCGCTGCTGTCAGTAACGACTTTGCTGCCGCAGGCGTTG
AAGTCACATCTTACGGATCTGGATCGCGAGTTCTTGCTCAGTTTTAAGCAAAACAGCCCTGACTGGTCGCGGTATCGCTA
TCCTGAAATTCAGCATCTTCCGGCTATTCGCTGGAAGCAACGTAATCTGGCGGTGCTGAAAGATAAAAATCCGGCGAAGT
ATGTGGCGGCGGTGAATAAGCTAGAGAGGGTGCTGGCGTAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT226886 WP_000948331.1 NZ_CP088158:c2081535-2081365 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT226886 NZ_CP122648:c4021474-4020692 [Escherichia coli]
TTGCCAAACTATCTAAACTGGTTGATGCAAAATACGCTACCCGGTCAGGTGCTGCTCCAGTCATGGCTTACCCGCAACGG
CGTAGATCGTAAGCTTTCTTATCTCTATGTTCAAAATGGCTGGCTCAGGCGCATCGCTCACGGCGTGTACTGCCGGACGG
GCCGTGAGCCGGATTGGGTAGACGCGGTTTACTGCCTGCAAACACAGTGGGAAAAGCCTGTTCGGGTGGCGGGGCTAACC
AGCCTTGCGCTACAGGGCCATGCGCACTATACCGAACCGGGCAGGCCGCAGGTCTGGCTCGCGCTTCCGGCTTACGTTAA
GCTGCCGGGATGGTTTGCTGCCTTCGAACAATCTGCAACATTTATCACGCTGTATACATCGGGGCTTACCGTAGATGTAA
CGTCATTGACTACGGAACTAAAAATTGGCGACTGCCAGTTAGCAGGCAGTGTTCCGGAACTGGCCGCGTATGAGATCGCC
TGCGGCGTTCCTTCGTTGATCTCGTTCGAACATGCCGATGCTCTGTTTCAGGGGTTGAATCTTCTCAGCCCACGCAAATT
GCAGGCACTGCTTCGCGCCAGTCATTCGGTTAAAACCAACCGGGTGATGCTTTACCTTGCCCGGCGTAATGGACACCCTT
ATTTTCAGTATATTGATCAAAGCGGTATAGAAACAGGTACGGGAAAGCGGCAAATCATACCCGGCGGCTGGCTGGAGCCG
GATTATCAAATTACTGTTCCACGCGCATTTAAACCGGAAGGAGTCGAAGATGGATCTGCGTGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1