Detailed information of TA system TA226873

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsdAT/-
• Location: 2185106..2185620
• Replicon: chromosome
• Accession: NZ_CP088157
• Organism: Staphylococcus aureus strain 199

Toxin (Protein)

• Gene name: TsdT
• Uniprot ID: -
• Locus tag: LQK05_RS11020
• Protein ID: WP_217654535.1
• Coordinates: 2185106..2185315 (-)
• Length: 70 a.a.

Antitoxin (Protein)

• Gene name: TsdA
• Uniprot ID: W8UW13
• Locus tag: LQK05_RS11025
• Protein ID: WP_000138932.1
• Coordinates: 2185327..2185620 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LQK05_RS11000 (2180614)	2180614..2181972	-	1359	WP_000611459.1	UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase	-
LQK05_RS11005 (2181987)	2181987..2183057	-	1071	WP_000159631.1	D-alanine--D-alanine ligase	-
LQK05_RS11010 (2183375)	2183375..2184577	+	1203	WP_001109936.1	FtsW/RodA/SpoVE family cell cycle protein	-
LQK05_RS11015 (2184841)	2184841..2184978	-	138	WP_000828354.1	Lmo0850 family protein	-
LQK05_RS11020 (2185106)	2185106..2185315	-	210	WP_217654535.1	heavy metal-associated domain-containing protein	Toxin
LQK05_RS11025 (2185327)	2185327..2185620	-	294	WP_000138932.1	copper-sensing transcriptional repressor CsoR	Antitoxin
LQK05_RS11030 (2185786)	2185786..2187270	+	1485	WP_000571549.1	cardiolipin synthase	-
LQK05_RS11035 (2187298)	2187298..2187945	+	648	WP_001187630.1	HD domain-containing protein	-
LQK05_RS11040 (2188411)	2188411..2189283	-	873	WP_000725802.1	membrane protein insertase YidC	-
LQK05_RS11045 (2189369)	2189369..2190010	-	642	WP_000483153.1	thiamine phosphate synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Heavy metal-associated domain, HMA (23-66)

Antitoxin

• Metal-sensitive transcriptional repressor (14-92)

Sequences

Toxin

Protein

• Length: 70 a.a.
• Molecular weight: 7834.76 Da
• Isoelectric Point: 3.9389

>T226873 WP_217654535.1 NZ_CP088157:c2185315-2185106 [Staphylococcus aureus]
MIHQNTIYTAGIETEEQVSQLTERISNMIGVQQVNINIIDGQVTVSYETPANLNSIEKEIYDEGYKIVF

Nucleotide

• Length: 210 bp

>T226873 NZ_CP122648:915518-915925 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATCTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATCCACGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGCATTAATGCTCGCCAGGGGGAAATTCGCCTGTTGATGGACGGGCGTTTGCGCTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGACAGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATATTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11197.82 Da
• Isoelectric Point: 6.2243

>AT226873 WP_000138932.1 NZ_CP088157:c2185620-2185327 [Staphylococcus aureus]
MTEQDNAHHSEQIKTNLKSRLNRIEGQVRAINRMIEEDVYCDDVLTQIRATRSALNSVAIKLLEQHMKSCIMNKVNQGAQ
EEAMEELLVTFQKLIKD

Nucleotide

• Length: 294 bp

>AT226873 NZ_CP122648:915271-915537 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAGTGTGACGATCCCGATC
TGTTTAACTGGCTGATGAACCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Antitoxin

• AlphaFold DB: W8UW13