Detailed information of TA system TA226872

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2165348..2165877
• Replicon: chromosome
• Accession: NZ_CP088157
• Organism: Staphylococcus aureus strain 199

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: LQK05_RS10930
• Protein ID: WP_000621177.1
• Coordinates: 2165348..2165710 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: LQK05_RS10935
• Protein ID: WP_000948331.1
• Coordinates: 2165707..2165877 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LQK05_RS10910 (2162326)	2162326..2163096	-	771	WP_001041107.1	RNA polymerase sigma factor SigB	-
LQK05_RS10915 (2163071)	2163071..2163550	-	480	WP_001190829.1	anti-sigma B factor RsbW	-
LQK05_RS10920 (2163552)	2163552..2163878	-	327	WP_001052491.1	anti-sigma factor antagonist	-
LQK05_RS10925 (2163997)	2163997..2164998	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
LQK05_RS10930 (2165348)	2165348..2165710	-	363	WP_000621177.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
LQK05_RS10935 (2165707)	2165707..2165877	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
LQK05_RS10940 (2165962)	2165962..2167110	-	1149	WP_001281147.1	alanine racemase	-
LQK05_RS10945 (2167176)	2167176..2167535	-	360	WP_000581202.1	holo-ACP synthase	-
LQK05_RS10950 (2167539)	2167539..2168030	-	492	WP_001205910.1	PH domain-containing protein	-
LQK05_RS10955 (2168017)	2168017..2169600	-	1584	WP_001294627.1	PH domain-containing protein	-
LQK05_RS10960 (2169593)	2169593..2170072	-	480	WP_001287078.1	hypothetical protein	-
LQK05_RS10965 (2170281)	2170281..2170841	-	561	WP_001092409.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13471.72 Da
• Isoelectric Point: 10.1654

>T226872 WP_000621177.1 NZ_CP088157:c2165710-2165348 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNTVAHQKN

Nucleotide

• Length: 363 bp

>T226872 NZ_CP122648:c832968-832591 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCACGCCCGTCTCCGGTTGAAATCTGGCAGACACTGCTCAC
CCGACTGCTGGATCAGCACTACGGCCTTACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGCGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTACGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGTTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCACGCCGGGCAACCGGCCTGATGACACGCGA
CAACTACAGAACGGTAAATAACATTACCCTGGGTAAACATCCGGGGGCGAAACAATGA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT226872 WP_000948331.1 NZ_CP088157:c2165877-2165707 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT226872 NZ_CP122648:c833426-833058 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACGCTTCCCGACGACAATAACGACCGCACCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTTGGTGCCCGTCTGGTACAGGAAGGCAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGGCGGT
TCAGCGACGCGGATTCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGTTG
TGGCTACGTTTATATGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1