Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PemK-MazE
Location 1182862..1183430 Replicon chromosome
Accession NZ_CP088151
Organism Mesorhizobium mediterraneum strain R31

Toxin (Protein)


Gene name mazF Uniprot ID -
Locus tag LRP31_RS05645 Protein ID WP_095487536.1
Coordinates 1183083..1183430 (+) Length 116 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID -
Locus tag LRP31_RS05640 Protein ID WP_164759292.1
Coordinates 1182862..1183086 (+) Length 75 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LRP31_RS05610 (LRP31_05605) 1177937..1178275 + 339 WP_095487715.1 cupin domain-containing protein -
LRP31_RS05615 (LRP31_05610) 1178580..1178723 + 144 WP_158302646.1 hypothetical protein -
LRP31_RS05620 (LRP31_05615) 1178723..1180561 + 1839 WP_095487545.1 DNA helicase RecQ -
LRP31_RS05625 (LRP31_05620) 1180646..1181035 - 390 WP_095487543.1 hypothetical protein -
LRP31_RS05630 (LRP31_05625) 1181168..1181527 + 360 WP_245461552.1 hypothetical protein -
LRP31_RS05635 (LRP31_05630) 1181592..1182737 + 1146 WP_095487540.1 hypothetical protein -
LRP31_RS05640 (LRP31_05635) 1182862..1183086 + 225 WP_164759292.1 antitoxin MazE family protein Antitoxin
LRP31_RS05645 (LRP31_05640) 1183083..1183430 + 348 WP_095487536.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
LRP31_RS05650 (LRP31_05645) 1183540..1183863 - 324 WP_095487534.1 hypothetical protein -
LRP31_RS05655 (LRP31_05650) 1183968..1184891 - 924 WP_095487532.1 alpha/beta hydrolase -
LRP31_RS05660 (LRP31_05655) 1185076..1186050 + 975 WP_095487713.1 Tim44 domain-containing protein -
LRP31_RS05665 (LRP31_05660) 1186075..1186716 - 642 WP_095487530.1 class I SAM-dependent methyltransferase -
LRP31_RS05670 (LRP31_05665) 1186928..1187110 - 183 WP_127205609.1 hypothetical protein -
LRP31_RS05675 (LRP31_05670) 1187300..1188016 - 717 WP_095487528.1 pentapeptide repeat-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(2-104)

Antitoxin

Antitoxin MazE-like

(13-74)


Sequences

Toxin        


Download         Length: 116 a.a.        Molecular weight: 12445.49 Da        Isoelectric Point: 6.2881

>T226852 WP_095487536.1 NZ_CP088151:1183083-1183430 [Mesorhizobium mediterraneum]
MKRGDIWTVAGGKDFAGKPRPIVIIQDDSFDATDSITICGLTTDETDAPLFRLAVEPNERNGLLAVCRLMVDKITTVPKS
KIGANIGRLDDADILRLNQAVIVFLGLAVSPRLKP

Download         Length: 348 bp

>T226852 NZ_CP122643:2027477-2027728 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCTGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Antitoxin


Download         Length: 75 a.a.        Molecular weight: 8267.27 Da        Isoelectric Point: 10.4473

>AT226852 WP_164759292.1 NZ_CP088151:1182862-1183086 [Mesorhizobium mediterraneum]
MASASKPKSSGMKVREHRERLRRQGLRPIQIWVPDVRAPAFRSEAHRQSLAVAASAHASEDQAFIDAISDWGDE

Download         Length: 225 bp

>AT226852 NZ_CP122643:2027725-2027979 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAGTCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References