Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1-VagC
Location 3450216..3450887 Replicon chromosome
Accession NZ_CP088147
Organism Mesorhizobium ciceri strain R30

Toxin (Protein)


Gene name vapC Uniprot ID -
Locus tag LRP29_RS16655 Protein ID WP_024501524.1
Coordinates 3450489..3450887 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A3S2HAG4
Locus tag LRP29_RS16650 Protein ID WP_024501525.1
Coordinates 3450216..3450488 (+) Length 91 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LRP29_RS16620 (LRP29_16585) 3445953..3446375 + 423 WP_024501529.1 DoxX family protein -
LRP29_RS16625 (LRP29_16590) 3446418..3447287 + 870 WP_024501528.1 NmrA family NAD(P)-binding protein -
LRP29_RS16630 (LRP29_16595) 3447402..3447851 - 450 WP_013530871.1 Rrf2 family transcriptional regulator -
LRP29_RS16635 (LRP29_16600) 3448108..3449094 - 987 WP_013530872.1 sulfate ABC transporter substrate-binding protein -
LRP29_RS16640 (LRP29_16605) 3449347..3449628 + 282 WP_024501527.1 DUF1778 domain-containing protein -
LRP29_RS16645 (LRP29_16610) 3449628..3450113 + 486 WP_024501526.1 GNAT family N-acetyltransferase -
LRP29_RS16650 (LRP29_16615) 3450216..3450488 + 273 WP_024501525.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
LRP29_RS16655 (LRP29_16620) 3450489..3450887 + 399 WP_024501524.1 type II toxin-antitoxin system VapC family toxin Toxin
LRP29_RS16660 (LRP29_16625) 3450902..3451942 - 1041 WP_024501523.1 sulfate/molybdate ABC transporter ATP-binding protein -
LRP29_RS16665 (LRP29_16630) 3451956..3452840 - 885 WP_013530874.1 sulfate ABC transporter permease subunit CysW -
LRP29_RS16670 (LRP29_16635) 3452833..3453684 - 852 WP_013530875.1 sulfate ABC transporter permease subunit CysT -
LRP29_RS16675 (LRP29_16640) 3453706..3454731 - 1026 WP_013530876.1 sulfate ABC transporter substrate-binding protein -
LRP29_RS16680 (LRP29_16645) 3454952..3455710 - 759 WP_245265283.1 M15 family metallopeptidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(4-122)

Antitoxin

SpoVT-AbrB domain

(17-54)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14169.36 Da        Isoelectric Point: 5.7431

>T226846 WP_024501524.1 NZ_CP088147:3450489-3450887 [Mesorhizobium ciceri]
MRFMLDTNIISDMIRNPAGKAARAMSREGDDAVCTSIVVASELRYGCARKGSAKLLKKVEDLLAEIPVLPLDVPVDAEYG
GLRAELEAAGQTIGHNDLFIAAHACALGATLVTANTGEFTRIRNLKVENWLA

Download         Length: 399 bp

>T226846 NZ_CP122641:c98270-98118 [Escherichia coli]
ATGCCACAGCGAACGTTTTTAATGATGTTAATCGTCATCTGTGTGACGATTCTGTGTTTTGTCTGGATGGTGAGGGATTC
GCTTTGCGTACTCCGGCTCCAGCAGGGAAACACAGTGCTTGTGGCAACGTTAGCCTACGAAGTTAAACGTTAA

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 10263.66 Da        Isoelectric Point: 4.9001

>AT226846 WP_024501525.1 NZ_CP088147:3450216-3450488 [Mesorhizobium ciceri]
MPQNRHSTTPPKEAKLFRNNRSQAVRIPVEFELPGEKVLISREGDRLVIEPVRKHGLTALLAQWAKEPPLDPEDDLPEID
DTPVKSEDIF

Download         Length: 273 bp

>AT226846 NZ_CP122641:98320-98382 [Escherichia coli]
TCGAAGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB A0A3S2HAG4

References