Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 2624761..2625389 Replicon chromosome
Accession NZ_CP088147
Organism Mesorhizobium ciceri strain R30

Toxin (Protein)


Gene name vapC Uniprot ID A0A436HUY3
Locus tag LRP29_RS12450 Protein ID WP_024504523.1
Coordinates 2625003..2625389 (+) Length 129 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID X6D5I3
Locus tag LRP29_RS12445 Protein ID WP_023760667.1
Coordinates 2624761..2625006 (+) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LRP29_RS12415 (LRP29_12395) 2620290..2621393 - 1104 WP_024504518.1 AbrB family transcriptional regulator -
LRP29_RS12420 (LRP29_12400) 2621485..2621730 - 246 WP_024504519.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
LRP29_RS12425 (LRP29_12405) 2621759..2622067 - 309 WP_024504520.1 putative quinol monooxygenase -
LRP29_RS12430 (LRP29_12410) 2622131..2622910 - 780 WP_024504521.1 SDR family oxidoreductase -
LRP29_RS12435 (LRP29_12415) 2623036..2623473 - 438 WP_013529935.1 DsrE family protein -
LRP29_RS12440 (LRP29_12420) 2623576..2624694 + 1119 WP_024504522.1 hypothetical protein -
LRP29_RS12445 (LRP29_12425) 2624761..2625006 + 246 WP_023760667.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
LRP29_RS12450 (LRP29_12430) 2625003..2625389 + 387 WP_024504523.1 type II toxin-antitoxin system VapC family toxin Toxin
LRP29_RS12455 (LRP29_12435) 2625396..2626343 - 948 WP_013529938.1 cation diffusion facilitator family transporter -
LRP29_RS12460 (LRP29_12440) 2626349..2628538 - 2190 WP_024504524.1 anthranilate synthase -
LRP29_RS12465 (LRP29_12445) 2628858..2629628 + 771 WP_024504525.1 isocitrate lyase/phosphoenolpyruvate mutase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(3-121)

Antitoxin

SpoVT-AbrB domain

(7-43)


Sequences

Toxin        


Download         Length: 129 a.a.        Molecular weight: 14314.44 Da        Isoelectric Point: 7.4028

>T226842 WP_024504523.1 NZ_CP088147:2625003-2625389 [Mesorhizobium ciceri]
VIYLLDTNAVIAVIKGDAGLLALLKRHRPQDFALSAIVAHELHYGAEKSQRRAENLARIEALQFPVLEFDREDARHAGEI
RATLATLGTPIGPYDALIGGQARARNLTLMTRNVREFERIKDLSIETW

Download         Length: 387 bp

>T226842 NZ_CP122639:21490-21741 [Escherichia coli]
ATGAGAAAAAATAAAGGTGATGTGACTTATTTTCTTGAAAAAGAAGGTGACAATTACAGGCTTACAAAAAGGATAAAAGC
CAGAACAAATGTTAAAATCGGGAACAAAACCACGAAAATTACATTGTATGATGCCGTACTGAATGAGAATGAATTACAAC
ATATTGATTTTACGTGTGCCGGATTAAGGGAAGACGATGAGACGCCAGTTAAGAATTTAATTAAGGAGTTTATGTTAAAT
GAAACCCGATAA

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 9370.49 Da        Isoelectric Point: 4.4734

>AT226842 WP_023760667.1 NZ_CP088147:2624761-2625006 [Mesorhizobium ciceri]
MDKAKVFWSGRSQAVRLPKEFRFETDEVSIRRHGQTVILEPLAQDWAWLDQVIGPLDNDFAEAALERQDGQDRPALDDVF
K

Download         Length: 246 bp

>AT226842 NZ_CP122639:21728-21955 [Escherichia coli]
ATGAAACCCGATAATACACCTGAAAATGTAAAAAAATTACGTTTAAAAGCAGGATTAACACAAAAGGAATGCAGTCATAT
TTATGGTGTTGGGCTAAGAACCTGGCAGAAAAAAGAGGAAGTTAATACTCAAAACAGTCAAAGTCTGTCACTGGTTGAGT
TTGAGTTTCTTTTGTTACTTGCAGGAGAACATCCTGAATACGTGCTCTGCAAAAGGGAGTCAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A436HUY3

Antitoxin

Source ID Structure
AlphaFold DB X6D5I3

References