Detailed information of TA system TA226840

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-pasA/RelE-HTH
• Location: 101046..101529
• Replicon: chromosome
• Accession: NZ_CP088145
• Organism: Corynebacterium diphtheriae strain SCPM-O-B-4453

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A806GHY4
• Locus tag: LRM94_RS00545
• Protein ID: WP_014302726.1
• Coordinates: 101266..101529 (+)
• Length: 88 a.a.

Antitoxin (Protein)

• Gene name: pasA
• Uniprot ID: -
• Locus tag: LRM94_RS00540
• Protein ID: WP_003850117.1
• Coordinates: 101046..101282 (+)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LRM94_RS00515 (LRM94_00515)	96425..96823	+	399	WP_003850107.1	LysE family transporter	-
LRM94_RS00520 (LRM94_00520)	97089..97847	+	759	WP_003850109.1	hypothetical protein	-
LRM94_RS00525 (LRM94_00525)	98127..98975	+	849	WP_003850110.1	helix-turn-helix domain-containing protein	-
LRM94_RS00530 (LRM94_00530)	98897..99649	+	753	WP_227889257.1	IS30 family transposase	-
LRM94_RS00535 (LRM94_00535)	99860..100543	-	684	WP_014302723.1	hypothetical protein	-
LRM94_RS00540 (LRM94_00540)	101046..101282	+	237	WP_003850117.1	ribbon-helix-helix domain-containing protein	Antitoxin
LRM94_RS00545 (LRM94_00545)	101266..101529	+	264	WP_014302726.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LRM94_RS00550 (LRM94_00550)	101656..101949	+	294	WP_014302727.1	RNA-binding S4 domain-containing protein	-
LRM94_RS00555 (LRM94_00555)	101949..102773	+	825	WP_014301205.1	VOC family protein	-
LRM94_RS00560 (LRM94_00560)	102779..103084	+	306	WP_003850125.1	MGMT family protein	-
LRM94_RS00565 (LRM94_00565)	103094..104014	-	921	WP_003850127.1	alpha/beta hydrolase	-
LRM94_RS00570 (LRM94_00570)	104154..104780	+	627	WP_014318472.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	67060..101949	34889

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

• Predicted DNA-binding protein, ribbon-helix-helix domain (7-43)

Sequences

Toxin

Protein

• Length: 88 a.a.
• Molecular weight: 9911.45 Da
• Isoelectric Point: 10.9201

>T226840 WP_014302726.1 NZ_CP088145:101266-101529 [Corynebacterium diphtheriae]
VAWEISFSPRAVKSFKKLDTGEQRRVSKFLREVGALEDPRLRGKALTANKSGLWRWRVGDYRIIADIVDARVVVVVVDVG
HRSKAYD

Nucleotide

• Length: 264 bp

>T226840 NZ_CP122638:11224-11709 [Escherichia coli]
ATGGGATGTGTAACAGCACCAGAACCTTTATCCTCATTTCATCAGGTCGCTGAATTTGTCAGCGGTGAAGCTGTTCTTGA
TGACTGGTTAAAGCAGAAGGGGCTAAAAAATCAGGCTCTCGGAGCCACCCGGACTTTTGTTGTATGCAGGAAAGGTACGC
AACAAGTAGTCGGTTTTTACTCACTGGCTACTGGCAGTGTCAACCATACAGAAGCTACAGGCAATCTTCGGCGCAATATG
CCTGACCCCATCCCTGTCATTATACTTGCACGACTGGCTGTTGATGCGTCATTCCGTGGTAAAGGGCTTGGTGCGGATTT
ACTACATGATGCAGTTCGTCGTTGTTACAGAGTGGCCGAAAATATCGGTGTACGTGCAATTATGGTTCATGCACTTACGG
AAAGCGCAAAACAGTTTTATATCCATCATGGCTTTACACCATCAAAAACTCAGCTGCAGACATTGTTTCTTAAACTCCCT
CAATAA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8911.02 Da
• Isoelectric Point: 4.8604

>AT226840 WP_003850117.1 NZ_CP088145:101046-101282 [Corynebacterium diphtheriae]
MSGSVISLRIDDEQKRRLDELSRRTGRPCSFYLREALSAHLADLEYVYRLEEEAAQVRRGELGTVPLSQLERECGLGD

Nucleotide

• Length: 237 bp

>AT226840 NZ_CP122638:10952-11236 [Escherichia coli]
ATGATATACGGAGGCTTTATGAAATCAGATGTTCAGCTCAACCTCAGAGCTAGAGAATCACAGCGGATACTCATTGATAC
CGCCGCAGAAATTCTTCATAAGTCACGTACAGACTTCATTCTTGAAATGGCCTGTAAAGCTGCGGAAGATGTTATTCTTG
ACCGGCGGGTGTTTAATTTTAATGACAGGCAATATGAAGAGTTCATCGAAATGCTTGATGCTCCGGTTGCAGATGAGCCT
GCCATCGAAAAATTACTGGCAAGGAAACCTCAATGGGATGTGTAA

Structures

Toxin

• AlphaFold DB: A0A806GHY4