Detailed information of TA system TA022684

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 1597905..1598543
• Replicon: chromosome
• Accession: NC_010473
• Organism: Escherichia coli str. K-12 substr. DH10B

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: ECDH10B_RS08040
• Protein ID: WP_000813794.1
• Coordinates: 1597905..1598081 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: ECDH10B_RS08045
• Protein ID: WP_001270286.1
• Coordinates: 1598127..1598543 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECDH10B_RS08020 (ECDH10B_1563)	1593524..1594738	-	1215	WP_001326689.1	BenE family transporter YdcO	-
ECDH10B_RS08025 (ECDH10B_1564)	1594791..1595327	+	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
ECDH10B_RS08030 (ECDH10B_1565)	1595400..1597361	+	1962	WP_001303492.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
ECDH10B_RS08035 (ECDH10B_1566)	1597453..1597683	-	231	WP_000494244.1	YncJ family protein	-
ECDH10B_RS08040 (ECDH10B_1567)	1597905..1598081	+	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
ECDH10B_RS08045 (ECDH10B_1568)	1598127..1598543	+	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
ECDH10B_RS08050 (ECDH10B_1569)	1598622..1600028	+	1407	WP_000760626.1	PLP-dependent aminotransferase family protein	-
ECDH10B_RS08055 (ECDH10B_1570)	1600273..1601418	+	1146	WP_000047424.1	ABC transporter substrate-binding protein	-
ECDH10B_RS08060 (ECDH10B_1571)	1601436..1602449	+	1014	WP_000220396.1	ABC transporter ATP-binding protein	-
ECDH10B_RS08065 (ECDH10B_1572)	1602450..1603391	+	942	WP_001251304.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T22684 WP_000813794.1 NC_010473:1597905-1598081 [Escherichia coli str. K-12 substr. DH10B]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T22684 NC_010473:1597905-1598081 [Escherichia coli str. K-12 substr. DH10B]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT22684 WP_001270286.1 NC_010473:1598127-1598543 [Escherichia coli str. K-12 substr. DH10B]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT22684 NC_010473:1598127-1598543 [Escherichia coli str. K-12 substr. DH10B]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• PDB: 6HPB