Detailed information of TA system TA226815

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-RelB
• Location: 310176..310762
• Replicon: chromosome
• Accession: NZ_CP088142
• Organism: Salmonella enterica subsp. enterica serovar Gallinarum strain SCPM-O-B-4548

Toxin (Protein)

• Gene name: doc
• Uniprot ID: A0A3V9X0E4
• Locus tag: LRM93_RS01415
• Protein ID: WP_000174964.1
• Coordinates: 310394..310762 (+)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: V7IU48
• Locus tag: LRM93_RS01410
• Protein ID: WP_001522145.1
• Coordinates: 310176..310397 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LRM93_RS01385 (305197)	305197..306306	+	1110	WP_000822976.1	high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK	-
LRM93_RS01390 (306366)	306366..307292	+	927	WP_000003007.1	high-affinity branched-chain amino acid ABC transporter permease LivH	-
LRM93_RS01395 (307289)	307289..308566	+	1278	WP_000803764.1	branched chain amino acid ABC transporter permease LivM	-
LRM93_RS01400 (308563)	308563..309330	+	768	WP_000082080.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG	-
LRM93_RS01405 (309332)	309332..310045	+	714	WP_000416114.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF	-
LRM93_RS01410 (310176)	310176..310397	+	222	WP_001522145.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
LRM93_RS01415 (310394)	310394..310762	+	369	WP_000174964.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
LRM93_RS01420 (311021)	311021..312337	+	1317	WP_000624755.1	sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB	-
LRM93_RS01425 (312442)	312442..313329	+	888	WP_000099303.1	sn-glycerol-3-phosphate ABC transporter permease UgpA	-
LRM93_RS01430 (313326)	313326..314171	+	846	WP_000572193.1	sn-glycerol-3-phosphate ABC transporter permease UgpE	-
LRM93_RS01435 (314173)	314173..315243	+	1071	WP_000907840.1	sn-glycerol-3-phosphate import ATP-binding protein UgpC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	283028..315980	32952

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (6-114)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-49)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13601.91 Da
• Isoelectric Point: 6.7252

>T226815 WP_000174964.1 NZ_CP088142:310394-310762 [Salmonella enterica subsp. enterica serovar Gallinarum]
MTLQLISAEEIIQFHDRLLRVTPGVTGMPDPGRAEALMYRVLNQIEYEGVTDVWLLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGISLAANPDFVDMTVDAAAGRLTLEQIVVRLRA

Nucleotide

• Length: 369 bp

>T226815 NZ_CP122633:25764-26042 [Escherichia coli]
ATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGATAGC
CGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAACCTGGCCGGGCAGCCGTATAAAGGCCGCAACGGTCGTG
TGGAAGGTACCCGGGAACTGGTTATTCACCCGCATTTTGTTCTGGTTTATGAGGTCGACAGCCAGTGGGGAAAAGTGTAT
ATCCTGCGAGTATTGCATACTGTACAGAAGTGGCCATAG

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8166.17 Da
• Isoelectric Point: 5.0775

>AT226815 WP_001522145.1 NZ_CP088142:310176-310397 [Salmonella enterica subsp. enterica serovar Gallinarum]
MRTVNYSEARQNLAEVLESAVTAGPVTITRRGHKSAVIISAEEFERYQTARMDDEFAAIMAVHGNELRELADK

Nucleotide

• Length: 222 bp

>AT226815 NZ_CP122633:25495-25764 [Escherichia coli]
ATGGCACAGGTTAATATGAGTTTAAGAATTGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGGCTCTCAGTTAATCCGGGATTTTATGCGCCAGACCGTGGAACGGCAGCATAATACCTGGTTCCGTGACCAGGTTG
AGGCAGGACGTCAGCAACTCGAGCGCGGCGATGTACTTCCCCATGACATGGTCGAATCTTCCGCTGCTGCATGGCGGGAT
GAAATGAGCAGGAAAGTCGCCGGTAAATGA

Structures

Toxin

• AlphaFold DB: A0A3V9X0E4