Detailed information of TA system TA226813

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 116562..117187
• Replicon: plasmid pB-4549_1
• Accession: NZ_CP088139
• Organism: Salmonella enterica subsp. enterica serovar Hissar strain SCPM-O-B-4549

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: LRM92_RS24595
• Protein ID: WP_000911322.1
• Coordinates: 116562..116960 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: LRM92_RS24600
• Protein ID: WP_000450265.1
• Coordinates: 116960..117187 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LRM92_RS24580 (LRM92_24575)	112753..113247	+	495	WP_010999946.1	entry exclusion protein	-
LRM92_RS24585 (LRM92_24580)	113283..114014	+	732	WP_000782438.1	conjugal transfer complement resistance protein TraT	-
LRM92_RS24590 (LRM92_24585)	114391..116553	+	2163	WP_000009318.1	type IV conjugative transfer system coupling protein TraD	-
LRM92_RS24595 (LRM92_24590)	116562..116960	-	399	WP_000911322.1	type II toxin-antitoxin system VapC family toxin	Toxin
LRM92_RS24600 (LRM92_24595)	116960..117187	-	228	WP_000450265.1	toxin-antitoxin system antitoxin VapB	Antitoxin

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	ant(2'')-Ia / sul1 / dfrA23 / blaOXA-1 / ant(3'')-Ia	rck / pefD / pefC / pefA / pefB / fdeC / spvC / spvB	1..125931	125931

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-51)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14887.13 Da
• Isoelectric Point: 8.5264

>T226813 WP_000911322.1 NZ_CP088139:c116960-116562 [Salmonella enterica subsp. enterica serovar Hissar]
MLKFMLDTNICIFTIKNKPASVRERFNLNQGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLDVLDYDTPAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVGGLRTEDWS

Nucleotide

• Length: 399 bp

>T226813 NZ_CP122631:58050-58328 [Escherichia coli]
ATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGATGGC
TGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAGCCTGGCCGGGCAGCCGTATAAAGGCCGCAACGGTCGTG
TGGAAGGTCCCCGGGAACTGGTTATTCACCCGCATTTTGTTCTGGTTTATGAGGTCGACAGCCAGTGGGGAAAAGTGTAT
ATCCTGCGCGTGTTGCATACTGCACAGAAGTGGCCATAG

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8509.49 Da
• Isoelectric Point: 4.5358

>AT226813 WP_000450265.1 NZ_CP088139:c117187-116960 [Salmonella enterica subsp. enterica serovar Hissar]
METSVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAGESWDEWFDGNNVSADFMDNREQPSVQERESF

Nucleotide

• Length: 228 bp

>AT226813 NZ_CP122631:57781-58050 [Escherichia coli]
ATGGCACAGGTTAATATGAGTTTAAGAATCGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGGCTCTCAGTTAATCCGGGATTTTATGCGCCAGACCGTTGAACGGCAGCATAATACCTGGTTCCGTGACCAGGTTG
CGGCAGGACGTCAGCAACTCGAGCACGGCGATGTGCTTCCCCATGACATGGTCGAATCCTCTGCTGCAGCATGGCGGGAT
GAAATGAGCAGGAAGGTTGCCGGTAAATGA