Detailed information of TA system TA226807

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 4155688..4156264
• Replicon: chromosome
• Accession: NZ_CP088138
• Organism: Salmonella enterica subsp. enterica serovar Hissar strain SCPM-O-B-4549

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: M7RG88
• Locus tag: LRM92_RS20435
• Protein ID: WP_001131963.1
• Coordinates: 4155977..4156264 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: B5R9R5
• Locus tag: LRM92_RS20430
• Protein ID: WP_000063142.1
• Coordinates: 4155688..4155990 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LRM92_RS20415 (4152198)	4152198..4154348	+	2151	WP_000379927.1	pyruvate/proton symporter BtsT	-
LRM92_RS20420 (4154443)	4154443..4154646	+	204	WP_000460616.1	YbdD/YjiX family protein	-
LRM92_RS20425 (4154657)	4154657..4155613	+	957	WP_000187839.1	GTPase	-
LRM92_RS20430 (4155688)	4155688..4155990	-	303	WP_000063142.1	BrnA antitoxin family protein	Antitoxin
LRM92_RS20435 (4155977)	4155977..4156264	-	288	WP_001131963.1	BrnT family toxin	Toxin
LRM92_RS20440 (4156533)	4156533..4157447	-	915	WP_128190849.1	restriction endonuclease	-
LRM92_RS20445 (4157645)	4157645..4161154	+	3510	WP_001043484.1	type I restriction-modification system endonuclease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (37-93)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11294.80 Da
• Isoelectric Point: 9.5894

>T226807 WP_001131963.1 NZ_CP088138:c4156264-4155977 [Salmonella enterica subsp. enterica serovar Hissar]
MPMEFEWDANKAKSNLRKHGVRFEDAVLVFDDPRHLSRQERYENGEYRWQTLGLVHGIVVILVAHSVRFESGFEVIRIIS
ARKADRKERNRYEHG

Nucleotide

• Length: 288 bp

>T226807 NZ_CP122629:3732578-3733120 [Escherichia coli]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATCGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGTAGCACG
GAAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCAGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAATTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCG
ACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11373.96 Da
• Isoelectric Point: 10.1293

>AT226807 WP_000063142.1 NZ_CP088138:c4155990-4155688 [Salmonella enterica subsp. enterica serovar Hissar]
MSMVKHKRGNASALSAQHEAELKALAKKSDDEIDYSDIPASEDGQWSEAVRGKFFRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLREQNKK

Nucleotide

• Length: 303 bp

>AT226807 NZ_CP122629:3732283-3732594 [Escherichia coli]
ATGCCTGGAAAAACAGCCACTCTACCCGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACTAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Toxin

• PDB: 7VD7

Antitoxin

• AlphaFold DB: A0A4D6PB01