Detailed information of TA system TA022679

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 418645..419263
• Replicon: chromosome
• Accession: NC_010473
• Organism: Escherichia coli str. K-12 substr. DH10B

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: -
• Locus tag: ECDH10B_RS02135
• Protein ID: WP_001290581.1
• Coordinates: 418645..418863 (-)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: S1PTH5
• Locus tag: ECDH10B_RS02140
• Protein ID: WP_000344800.1
• Coordinates: 418889..419263 (-)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECDH10B_RS02105 (ECDH10B_0409)	413934..414506	+	573	WP_000779842.1	YbaY family lipoprotein	-
ECDH10B_RS02110 (ECDH10B_0410)	414537..414848	-	312	WP_000409911.1	MGMT family protein	-
ECDH10B_RS02115 (ECDH10B_0412)	415227..415580	+	354	WP_000878140.1	DUF1428 domain-containing protein	-
ECDH10B_RS02120 (ECDH10B_0413)	415622..417172	-	1551	WP_001310610.1	cyclic-guanylate-specific phosphodiesterase PdeB	-
ECDH10B_RS02125 (ECDH10B_0414)	417336..417806	-	471	WP_000136192.1	YlaC family protein	-
ECDH10B_RS02130 (ECDH10B_0415)	417922..418473	-	552	WP_000102564.1	maltose O-acetyltransferase	-
ECDH10B_RS02135 (ECDH10B_0416)	418645..418863	-	219	WP_001290581.1	hemolysin expression modulator Hha	Toxin
ECDH10B_RS02140 (ECDH10B_0417)	418889..419263	-	375	WP_000344800.1	Hha toxicity modulator TomB	Antitoxin
ECDH10B_RS02145 (ECDH10B_0418)	419809..422958	-	3150	WP_001132469.1	multidrug efflux RND transporter permease subunit	-
ECDH10B_RS02150 (ECDH10B_0419)	422981..424174	-	1194	WP_001295324.1	multidrug efflux RND transporter periplasmic adaptor subunit AcrA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-117)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8678.06 Da
• Isoelectric Point: 8.9008

>T22679 WP_001290581.1 NC_010473:c418863-418645 [Escherichia coli str. K-12 substr. DH10B]
MSEKFLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Nucleotide

• Length: 219 bp

>T22679 NC_010473:c418863-418645 [Escherichia coli str. K-12 substr. DH10B]
ATGTCCGAAAAATTTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 14557.39 Da
• Isoelectric Point: 4.7395

>AT22679 WP_000344800.1 NC_010473:c419263-418889 [Escherichia coli str. K-12 substr. DH10B]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Nucleotide

• Length: 375 bp

>AT22679 NC_010473:c419263-418889 [Escherichia coli str. K-12 substr. DH10B]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Antitoxin

• AlphaFold DB: A0A7U9QBQ5