Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 11716..12371 Replicon plasmid pB-4515_1
Accession NZ_CP088137
Organism Salmonella enterica subsp. enterica serovar Typhimurium strain SCPM-O-B-4515

Toxin (Protein)


Gene name vagD Uniprot ID H9AC96
Locus tag LRM91_RS22535 Protein ID WP_001746752.1
Coordinates 11716..12144 (-) Length 143 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID H9AC95
Locus tag LRM91_RS22540 Protein ID WP_001261283.1
Coordinates 12141..12371 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LRM91_RS22500 (LRM91_22500) 7117..7881 + 765 WP_249895817.1 fimbrial protein -
LRM91_RS22505 (LRM91_22505) 7868..8143 + 276 WP_001020576.1 hypothetical protein -
LRM91_RS22510 (LRM91_22510) 8143..8856 + 714 WP_000801114.1 hypothetical protein -
LRM91_RS22515 (LRM91_22515) 8981..9565 + 585 WP_024134326.1 hypothetical protein -
LRM91_RS22520 (LRM91_22520) 9638..9850 + 213 WP_000063791.1 FaeA/PapI family transcriptional regulator -
LRM91_RS22525 (LRM91_22525) 10111..10272 + 162 WP_001816720.1 hypothetical protein -
LRM91_RS22530 (LRM91_22530) 10298..11146 + 849 WP_249895818.1 SdiA-regulated domain-containing protein -
LRM91_RS22535 (LRM91_22535) 11716..12144 - 429 WP_001746752.1 type II toxin-antitoxin system VapC family toxin Toxin
LRM91_RS22540 (LRM91_22540) 12141..12371 - 231 WP_001261283.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
LRM91_RS22545 (LRM91_22545) 13116..13334 + 219 WP_000813641.1 type II toxin-antitoxin system antitoxin CcdA -
LRM91_RS22550 (LRM91_22550) 13336..13641 + 306 WP_001159863.1 type II toxin-antitoxin system toxin CcdB -
LRM91_RS22555 (LRM91_22555) 13643..13933 + 291 WP_001266176.1 hypothetical protein -
LRM91_RS22560 (LRM91_22560) 13930..14451 + 522 WP_249895819.1 cytoplasmic protein -
LRM91_RS22565 (LRM91_22565) 14486..15268 + 783 WP_249895820.1 site-specific integrase -
LRM91_RS22570 (LRM91_22570) 15277..15828 + 552 WP_000545754.1 EAL domain-containing protein -
LRM91_RS22575 (LRM91_22575) 16015..16503 + 489 WP_001075507.1 CaiF/GrlA family transcriptional regulator -
LRM91_RS22580 (LRM91_22580) 16497..16982 + 486 WP_000905606.1 membrane protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - faeH / faeI / fdeC / spvC / spvB 1..86811 86811


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(9-133)

Antitoxin

SpoVT-AbrB domain

(7-50)


Sequences

Toxin        


Download         Length: 143 a.a.        Molecular weight: 15483.07 Da        Isoelectric Point: 8.1325

>T226786 WP_001746752.1 NZ_CP088137:c12144-11716 [Salmonella enterica subsp. enterica serovar Typhimurium]
MKQPRTKTYMLDTCICSFIMREKSEAVLKRLEQAVLRGHRIVISAITYSEMRFGATGPKASPRLVQLVDAFCARLDAILP
WDRAAVDATTEIKVALCLAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVS

Download         Length: 429 bp

>T226786 NZ_CP122625:c4027509-4027222 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGAAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTATTGTTTGATGATCCACAACATTTGTCACAGCAAGAACGCATAGAAAACGGTGAGTATCGCTGGCAGACAATCGGCC
TGGTTTACGGCATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8737.83 Da        Isoelectric Point: 4.3775

>AT226786 WP_001261283.1 NZ_CP088137:c12371-12141 [Salmonella enterica subsp. enterica serovar Typhimurium]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQLEKADPDFMAEREDVVSDEGRFEL

Download         Length: 231 bp

>AT226786 NZ_CP122625:c4027235-4026933 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCGCTGGCGAA
TAAATCCGACGAAGATATTGATTACAGTGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCCGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A764IXM1

Antitoxin

Source ID Structure
AlphaFold DB A0A6X6R7C5

References